Vitamin B12 [C61-64H84-90N14O13-14P ] Co.

Introduction Pernicious anemia, a megaloblastic anemia associated with neurological deterioration caused by progressive mylelination of nervous tissue was fatal till 1926. Then liver extract showed to be curative. subsequent work showed that liver contains an extrinsic factor in the form of Vit. B-12 . Intrinsic factor is also required which is produced by the body, which helps in its absorption.

IMPORTANT FUNCTIONS The most well-known function of B12 is its role in the development of red blood cells. As red blood cells mature, they require genetic information provided by DNA. Without B12, synthesis of DNA becomes defective, and so does the information needed for RBCs formation.

R= CN,CH3,OH, Deoxyadenosyl Dimethylbenzimidazol

CHEMISTRY Vitamin B12 includes a group of cobalt, containing compounds known as cobalamins. The major forms found in plasma and tissue include Methyl cobalamin Circulatory form Deoxyadenocyl cobalmin ( storage Form) and Hydroxy and cynao cobalamin (Supplemental form) All of these forms are biologically active.

Different forms of Vitamin B12 In food vit B12 is attached to protein in deoxyadenosyl form. To be utilized it is released by acid hydrolysis in the stomach or trypsin digestion in intestine. Then it combines with the Intrinsic Factor (IF), a protein secreted by the stomach which acts as carrier to ileum for absorption.

Stability and solubility Resistant to heat in aq.medium Slightly soluble in water Little lost during cooking Destroyed by strong acid and Alkalies

Food sources Vitamin B12 occurs only in animal sources. The richest sources include Lamb and calf beef Kidney Liver and Brain.

Other good sources include Heart Egg yolk and Variety of sea foods

Recommended Dietary Allowances 3.0 g of Vitamin B12 for adults allows for maintenance of adequate nutrition and substantial reserve body pool.

Absorption and Metabolism Vitamin B12 is absorbed through receptor sites in the ileum, mediated by intrinsic factor which is produced by glands in stomach. Vitamin is then transported across the intestinal cell and then into blood stream. Vitamin B12 is normally stored in liver for 3 to 5 years.

IMPORTANT FUNCTIONS Vitamin B12 is required by all cells of the body, especially Gastro-intestinal tract Bone marrow and Nervous system. Within bone marrow, it is involved in conversion of ribose nucleotides into deoxyribose nucleotides

How Deficiency develops ? Deficiency of Vitamin B12 is usually caused by defect in absorption rather than by dietary factors When intrinsic factor is not produced, Vit B12 is not absorbed.

As a result The cells become oversized and poorly shaped, begin to function ineffectively and a condition called pernicious anemia develops. Sometimes pernicious anemia isn't caused by a lack of B-12 itself, but by a lack of intrinsic factor, (protein) required for the absorption of B-12. Bone marrow cannot produced mature red blood cells and so releases the large, immature precursor (macrocytes) into circulation.

Effect on nerve cells A second major function of B12, less clearly understood than the first, involves its participation in the development of nerve cells. A coating which encloses the nerves, the myelin sheath is formed less successfully whenever B-12 is deficient. Abnormality in fatty acid synthesis.

Role in treating nervous system disorders Although the vitamin plays an indirect role in this process, supplementation of B12 has shown to be effective in relieving pain and other symptoms in a variety of nervous system disorders.

Other roles for vitamin B12 Protein in food required for growth and repair of cells depends upon B-12 for proper cycling through the body. Many of protein's key components( amino acids) become unavailable for use in the absence of B12.

Other roles for vitamin B12 Since a steps in CHO and fat metabolism requires B12 for its completion, deficiency of the vitamin can affect the metabolism of CHO and fats in the body.

Symptoms of megalotblastic anemia The symptoms of megaloblastic anemia include : Pallor Weight loss Anorexia Glossitis Sprue, and in advanced stages Degeneration of spinal cord.

FUNCTIONS B12 participates in two important chemical reactions in human body. Metionine synthase catalysed conversion of homocystein to methionine. 2. The 5-deoxyadenosyl derivatives of B12 is required for methyl malonyl-CoA mutase which converts methylmalonyl CoA to succinyl coA.

Contd: This is a key reaction in the catobolism of Valine, isoleucine,methionine,threonine, odd chain fatty acids, thymine and side chain of cholesterol. As expected B12 deficiency causes accumulation of both homocystein and methylmalonic acid.

B-12 deficiency ,if present The megaloblastic anemia Is due to the effect of B12 on folate metabolism. Methionine synthesis Homocysteine + N5 methyl THF → Methionine + THF Is the only pathway by which N5 – methyl THF can return to the tetrahydrofolate pool. Thus, in B12 deficiency essentially all of the folate becomes “trapped” as the N5 methyl derivative, causing a build up of N5 – methyltetrahydrofolate

THF, if deficient Tetrahydrofolate derivatives are needed for purine biosynthesis. By replenishing the THF pool , large amounts of supplemental folate can overcome the megaloblastic anemia, but not the neurolagical problems. This is the crux of the current debate on the optimal levels for folate fortication of foods. It is the megaloblastic anemia that usually brings the patient into the doctor’s office.

Thus, by masking the anemia, routine fortification of foods with high levels of folate could prevent detection of B12 deficiency until the neurological damage had become irreversible.

It has been proposed that the demyelination associated with B12 deficiency is caused by methylmalonyl CoA accumulation in two ways. (1) methylomalonyl CoA is a competitive inhibitor of malonly CoA in fatty acid biosynthesis. Because the myelin sheath is continually running over, any sever inhibition of fatty acid biosynthesis will lead to its degeneration.

(2) Methylmalonyl CoA can substitute for malonyl CoA in fatty acid synthesis leading to synthesis of branched-chain fatty acids, which may disrupt membrane structure. However the neurologic symptoms of B12 deficiency cannot be fully explained by either mechanism, since accumulation of both methylmalonic acid and homocysteine is required for demyelination.

Recent studies have shown that B12 deficiency is associated with increased expression of tumor necrosis factor α (TNF- α) and nerve growth factor (NGF) and decreased expression of epidermal growth factor (EGF) and interleukin in cerebrospinal fluid (CSF), but the mechanism of these changes and their effect on neural function remain unknown.

Toxic Effect No toxic effect in humans