Library # ESTs and Descriptions 5564 - Bacterial infected leaf 3982 - Flower bud 1336 -- Anther 5934 -- Fruit, early fruit 4240 -- Hair root 5211 -- Early root 6246 -- Placenta 5042 -- Pytophthora capsici infected plant 5804 -- Fruit,immature:1.5-7.5cm(+seeds) 5926 -- Fruit, mature green: pericarp(-seeds) 5951 -- Fruit, breaker: pericarp(-seeds) 5938 -- Fruit, mature red ripe: pericarp(-seeds) 5812 -- Seeds of friuts with placenta 5734 -- Flower bud, size 3~ 8mm 5735 -- Open flower 5668 -- Bark 5823 -- Peduncle 6833 -- Callus/ regeneration 5841 -- Germination stage: 4 & 6 day 5843 -- Germination stage: 18 day 5625 -- rbcS-silenced leaves 9749 Capsicum annuum 387 -- Capsicum chinense 162 -- Capsicum frutescens 16 -- Capsicum baccatum 9 -- Capsicum chacoense 6 -- Capsicum pubescens 5 -- Capsicum eximium 3 -- Capsicum ciliatum 3 -- Capsicum cardenasii 2 -- Capsicum galapagoense 2 -- Capsicum lycianthoides 2 -- Capsicum tovarii 1 -- Capsicum flexuosum 1 -- Capsicum spp.

Pepper Chip: Custom Content Capsicum ESTs 123,489 Capsicum genomic DNA 466 Capsicum mRNAs 515 COS markers 642 30,815 unigenes

Chip Design Array Format: 49 Feature Size: 5 µm Custom content:    6,415,642 probes/30,815 unigenes Background probes:    33,886 antigenomic (surrogate MM) probes Bacterial Expression sets: 81 probe sets including bioB, bioC, bioD from E. coli and cre from P1 Bacteriophage and Dap, lys, phe, thr, trp from B. subtilis Technical Replicate Controls: 100 probes organized into 10 sets of 10 probes each tiled 169 times SNP training set

Distribution of probes per sequence Mean =210 probes/unigene

Pepper Microarray Consortium PIs: Allen Van Deynze, Alex Kozik, Jiquiang Yao and Jim Prince Collaborators: Molly Jahn, Shanna Moore, Doil Choi                                             zx