Biological Forensics

Blood Artery White blood cells Platelets Red blood cells

Blood Plasma-55% Buffy coat-<1% Formed elements-45%

ANTIGENS

ANTIGENS b a b a b b a a b a b a b b a a

AGGLUTINATION

Blood being tested Serum Anti-A Anti-B Type AB (contains agglutinogens A and B; agglutinates with both sera) RBCs Type A (contains agglutinogen A; agglutinates with anti-A) Type B (contains agglutinogen B; agglutinates with anti-B) Type O (contains no agglutinogens; does not agglutinate with either serum) Figure 17.16

Blood Typing Serum A Serum B Serum D=Rh

What Blood Type is this Person?

Blood Type & Rh How Many Have It Frequency O Rh Positive 1 person in 3 37.4% Rh Negative 1 person in 15 6.6% A 35.7% 1 person in 16 6.3% B 1 person in 12 8.5% 1 person in 67 1.5% AB 1 person in 29 3.4% 1 person in 167 .6%

In Class Assignment Analyze 4 suspects blood types Compare it to blood found on the murder weapon. Determine the murderer.

Normal Polycythemia Anemia Sickle cell anemia

White Blood Cells Neutrophil 40-70% Monocyte 3-8%% Basophil <1% Lymphocyte 20-40% Eosinophil 2-4%

Identify WBC’s

Hematocrit Count Capillary tube

Hematocrit Count Capillary tube

Hematocrit Count clay Capillary tube

Hematocrit Count

Normal Values Male: 40-54 Female: 37-47 Red line 100 mm 49 mm clay