Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet.

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Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet Digital PCR Masaru Watanabe, Tomoya Kawaguchi, Shun-ichi Isa, Masahiko Ando, Akihiro Tamiya, Akihito Kubo, Hideo Saka, Sadanori Takeo, Hirofumi Adachi, Tsutomu Tagawa, Osamu Kawashima, Motohiro Yamashita, Kazuhiko Kataoka, Yukito Ichinose, Yukiyasu Takeuchi, Katsuya Watanabe, Akihide Matsumura, Yasuhiro Koh EBioMedicine Volume 21, Pages 86-93 (July 2017) DOI: 10.1016/j.ebiom.2017.06.003 Copyright © 2017 The Authors Terms and Conditions

Fig. 1 Multiplex assay for analysis of EGFR mutations. Two-dimensional histogram of the 6-hexaplex assay is shown. Plasmids containing each target sequence were encapsulated in droplets and subjected to the ddPCR assay. FAM, 6-carboxyfluorescein; TET, tetrachlorofluorescein. EBioMedicine 2017 21, 86-93DOI: (10.1016/j.ebiom.2017.06.003) Copyright © 2017 The Authors Terms and Conditions

Fig. 2 Serial dilutions of positive controls were analyzed for each EGFR mutation under study. Testing 0.04 and 4ng of genomic DNA from H1975 cells harboring EGFR mutations L858R and T790M (A) or PC-9/ZD cells harboring EGFR exon 19 deletion and substitution T790M (B); n=5. EBioMedicine 2017 21, 86-93DOI: (10.1016/j.ebiom.2017.06.003) Copyright © 2017 The Authors Terms and Conditions

Fig. 3 Comparison of results obtained by multiplex and duplex analyses. Compilation of the analysis of FFPE samples from patients with primary tumors mutated for EGFR L858R, Exon19 deletion (Ex19del) with/without T790M mutation. EBioMedicine 2017 21, 86-93DOI: (10.1016/j.ebiom.2017.06.003) Copyright © 2017 The Authors Terms and Conditions