Bioinformatics core facility, OUS/UiO

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Presentation transcript:

Bioinformatics core facility, OUS/UiO RNA-seq pipeline MBV3070 18th April, 2016 Ståle Nygård Bioinformatics core facility, OUS/UiO staaln@ifi.uio.no

Gene expression Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.

RNA sequencing (technology)

RNA sequencing (technology)

ELIXIR Galaxy RNA-seq pipeline At galaxy-ntnu.bioinfo.no Pipeline Tophat2 (alignment) FeatureCounts DESeq (differential expression)

DESeq bulids on negative binomial distribution

Estimating dispersion