Confirmation by real-time RT-PCR of selected genes differentially expressed in trout swim-up fry (A), juvenile brain (B) and liver (C) based on early nutritional.

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Confirmation by real-time RT-PCR of selected genes differentially expressed in trout swim-up fry (A), juvenile brain (B) and liver (C) based on early nutritional exposure to plant-based diet. Bars represent mean ± standard deviation of four fish, asterisks indicate significant (p < 0.05) differences. The fold change as identified by microarray analysis is outlined in the accompanying table. The genes chosen for the swim-up fry were keratin 13 (K13), Lissencephaly-1 homolog B (pafah1b1b), purpurin (rbp4l) and chymotrypsin B (Ctrb1). The genes chosen for the brain were Glucagon-2 (gcg2), purpurin (rbp4l), somatolactin (SL), Recoverin (Rcvrn) and cholecystokinin-Thr (CCK-T). The genes chosen for the liver were chymotrypsin B (Ctrb1), keratin 13 (K13) and cytosolic sulfotransferase 3 (ST1S3). The data from microarray experiments for probe expression of the corresponding genes is included in the table below the graphs.

A. Confirmation of selected microarray targets in trout swim-up fry by real-time PCR RT-qPCR data Microarray data

B. Confirmation of selected microarray targets in brain of juvenile trout by real-time PCR RT-qPCR data Microarray data

C. Confirmation of selected microarray targets in liver of juvenile trout by real-time PCR RT-qPCR data Microarray data