Preparation and Characterization of Beta-glucan/silica Nanocomposites Jung Ju Kim*1, Ji Hwa Beak2, Yangkyu Ahn1 1Department of Nanochemistry & Biochemistry, Konyang University Nonsan, Chungnam, Korea 320-711 2Department of chemistry, Konyang University
Abstract DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY A new class of beta-glucan/silica nanocomposites using a silica sol from water glass was successfully prepared by the sol-gel process. Silica particles with diameter of around 5 - 10 micrometer were observed by optical microscopy and scanning electron microscopy. FT-IR analyses confirmed that the composite microsphere was a segmented hybrid material composed of the beta-glucan segment and silica. The physicochemical properties such as morphological change by degradation, beta-glucan loading efficiency, and beta-glucan release profiles were investigated with the beta-glucan/silica nanocomposite microspheres. The speed of degradation and beta-glucan release decreased with increasing silica content. DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Objectives of this study Synthesis of decrease the molecular weight method of beta-glucan Synthesis of beta-glucan microsphere using crosslinking reaction Control of the size of the beta-glucan particles Synthesis of biopolymer-coated durgs microspheres for biomedical application DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
<structure of β-1,3-1,6-glucan> What is β-glucan? <structure of β-1,3-1,6-glucan> Safety of beta-glucan Beta-1,3-D glucan ㆍis a natural product ㆍhas been part of the human diet for thousands of years. ㆍis permitted for use in food by the U.S. FDA (GRAS) * GRAS : Generally Recognized As Safe Functional of beta-glucan - Immunotherapy of cancer - Immunity boosting - Counteraction of side-effects caused by cancer therapy - Prevention of infection - Wound healing &Skin regeneration - Cholesterol lowering - Recovery after radiation exposure character of beta-glucan - extraction in mushroom - Molecular form : (-C6H10O5-)n - Dissolved in alkali solution (NaOH solution) - Gelation in acid solution (acetic acid) Viscosity Emulsification activity DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Health assistance food and cosmetic Uses of β-glucan A field of study Use Medical supplies immunity reinforce Reinforcement of phagocytosis of macrophage to tuberculosis bacilli - resistance increase of a host Health assistance food and cosmetic immunity reinforcement material blood sugar and reinforcing agent of blood sugar - regrowth of the skin and anti-aging DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Molecular structures is β-Glucan DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Synthesis of the molecular weight method of beta-glucan )) Concentration of Beta-glucan solution )) Formic acid 80~90℃ Beta-glucan 1% beta-glucan 2% beta-glucan . 6% beta-glcuan 7% beta-glucan 1% beta-glucan 2% beta-glucan . 6% beta-glcuan 7% beta-glucan optimum conditions: solution in formic acid irradiation time : 20min irradiation temperature : keeping 80 ~ 90℃ temperature of Formic acid Under 80 ℃ Between 80 ℃ and 100 ℃ Over 100 ℃ Indissoluble Soluble evaporation of formic acid DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
+ Synthesis of beta-glucan microsphere using crosslinking reaction Volume of Span80 : 1ml, 2ml,···, 6ml, 7ml add glutaraldehyde iso-octane 35ml + span 80 5ml )) )) + Emulsification add Norfloxacin irradiation time : 20min irradiation time : 60min beta-glucan solution beta-glucan using crosslinking reaction Acetone +Alcohol(5:5) optimum conditions: solution in formic acid 5㎖ add durg : Norfloxacin irradiation time : 20min irradiation temperature : keeping 80 ~ 90℃ optimum conditions: solution in formic acid 5㎖ add durg : Norfloxacin irradiation time : 20min irradiation temperature : keeping 80 ~ 90℃ washing by centrifuge freeze drying DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Size distribution of beta-glucan by concentration of beta-glucan 1% 2% 3% 4% 5% 6% 7% Beta-glucan particle size (㎛) 0.252 (100%) 0.380 (87.9%) 0.500 (91.9%) 0.553 (85.4%) 0.913 (83.1%) 1.271 (92.2%) 1.979 (69.7%) S.Dev (nm/%) 0.047 0.116 0.172 0.308 0.445 0.252 0.911 DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Beta-glucan particle size (㎛) Size distribution of beta-glucan microsphere by volume of sulfactant Surfactant Concentant (vol.%) Beta-glucan particle size (㎛) S.Dev (nm / %) 0.079 0.416 0.089/18.07 0.103 0.747 0.425/10.43 0.146 1.335 0.589/14.95 0.167 2.827 1.355/4.92 DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
SEM image of beta-glucan was made to low molecule SEM image of before crosslinking reaction and after crosslinking reaction Before decrease the molecular weight method Before crosslinking reaction After decrease the molecular weight method After crosslinking reaction DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Beta-glucan image by sol-gel method DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Drug loading efficiency of beta-glucan Expression of drug loading efficiency of beta-glucan Drug contents : (volume of drugs within the microsphere) volume of drugs of microsphere + volume of beta-glucan ×100 ultrasonicator micro tip Beta-glucan microsphere 2㎎ Ethanol 10㎖ measurement : using UV spectrophotometer absorption of UV spectrophotometer : 205nm irradiation temperature : keeping 37.0±0.5℃ DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Conclusions Beta-glucan of high polymer was made to low molecule using treat by acid The beta-glucan microsphere were coated dugs by cross-linked method The size of particles was different by volume of surfactant According to the concentration of beta-glucan increases, the size of beta-glucan microsphere increases too According to the concentration of beta-glucan increases, The content of a medicine increased DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
Beta-glucan image by sol-gel method DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY