The Community of Practices ‘‘ Concept Applied to Rice Production in the Mekong Region: Quick Conversion of Popular Rice Varieties with Emphasis on Drought,

Slides:



Advertisements
Similar presentations
New Developments in Tall Fescues and Use Strategies.
Advertisements

Vision and Missions of the Turkey’s Seed Sector Kazım Abak 2 nd International Workshop on Seed Business Antalya 2-3 December 2013.
The community of practices: Rice Breeding in Mekong Region.
Planning rice breeding programs for impact
Marker Aided Selection for cooking quality and drought tolerance in Cambodia By Heang Dany and Then Rathmuny Cambodian Agricultural Research and Development.
Improvement of Rainfed Lowland Rice Variety for Cooking Quality Traits using MAS Sureeporn Katengam Uraiwan Kotchasatit UBONRATCHATHANI, THAILAND.
Biography Ashfaq M graduated from Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan in He visited McGill.
PROPOSAL Improvement of Rice Varieties Using Marker-Assisted Selection In Lao PDR.
6 Mark Tester Australian Centre for Plant Functional Genomics University of Adelaide Research developments in genetically modified grains.
Marker-assisted backcrossing for submergence tolerance
Rice standards pure Kenyan Pishori Anne kimani. overview Qualities of basmati Basmati in the world market Factors affecting quality Causes of loss of.
Mohammad Abd Elgawad Emam Assistant Lecturer, Agronomy Department,Faculty Of Agriculture.
In Vitro Screening. "The right to search for truth implies also a duty; one must not conceal any part of what one has recognized to be true." Albert Einstein,
Marker assisted breeding for striga resistance in sorghum in Eritrea
New technologies to save water for irrigated rice in the Sahel Michiel de Vries, Vincent Bado and Abdoulaye Sow Africa Rice Center (WARDA) Sahel Station.
GENOMIC MAPPING FOR DROUGHT TOLERANCE IN SORGHUM Introduction Drought is a major abiotic factor limiting crop production. Sorghum is one of the most drought.
Planning rice breeding programs for impact Factors affecting the adoption of improved varieties.
The Community of Practices “Concept applied to rice production in the Mekong Region: Quick conversion of popular rice varieties with emphasis on drought,
Development of Salt Tolerance in Rice Through Marker Assisted Selection Tin Tin Myint, and Thida Department of Agricultural Research (DAR), Yezin, Nay.
Providing Gender and Equity Balance in the NAPCC on Agriculture SUMAN SAHAI Gender and Economic Policy Discussion Forum, Inst. of Social Studies Trust.
Breeding for cooking quality with drought tolerance in rice by using Marker Assistant Selection By Heang Dany and Then Rathmuny Cambodian Agricultural.
Biotechnology - Agriculture And Food. Food problems have been a challenge to man since before we kept records. By the mid-1960’s, hunger and malnutrition.
PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF KENYAN BASMATI RICE VARIANT LINES: AN APPROACH TO GERMPLASM IMPROVEMENT AND PRESERVATION FAITH M. MWENDWA,
Plant biotechnology, one of the sections of biotechnology, Seed Bank and plant protection Division at Agricultural Research Department (ARD) in Myanmar.
What are the problems? Lower yield potential of varieties
Acknowledgements This study was performed with financial support of EEA grant EEZ08AP-27 and European Social Fund co-financed project 2009/0218/1DP/ /09/APIA/VIAA/099.
Improvement of Rainfed Lowland Rice Variety for Cooking Quality Traits using MAS Sureeporn Katengam Uraiwan Kotchasatit UBONRATCHATHANI, THAILAND.
Sequencing the genomes of a number of native Vietnamese rice lines Drought tolerant rice Salt tolerant rice Blight bacterial Blast rice Brownplanthopper.
APPLIED CONCEPTS IN PLANT BREEDING
Barley Research in TIAR Meixue Zhou. Barley Research in TIAR s Breeding –Long season and high rainfall areas - Franklin –Spring sown barley – Vertess.
In Vitro Selection Screen a large number of plants or cells for a certain characteristics.
PLAN Cooking Quality and Drought Improvement of Rice Kongpanh Kanyavong & Chanthakhone Boualaphanh The Agriculture Research Center, NAFRI, Ministry of.
Evaluation of advanced Cowpea lines for agronomic traits, yields, and response to cowpea scab caused by Sphaceloma spp. Dry Land Legume Research Program.
IRRI-Myanmar partnership: current and future perspectives for rice breeding RK Singh Rice Breeder IRRI, Philippines.
RESEARCH FOR DEVELOPMENT IN-HOUSE REVIEW 2012 Stress Tolerant Rice for Africa and South Asia (PVS on Rice varieties tolerant to Fe Toxicity) E. Annan-Afful.
Overview What is Plant Breeding? Basic Genetics Mendelian Genetics
Advancing the production of maize haploids in vivo RESULTS AND DISCUSSION Manifestation of the main inducers’ marker, R1-nj, has been improved in PHI lines.
Improving on TDK1-Aroma by Marker Assisted Selection (MAS)
DEVELOPMENT OF EXTRA-EARLY, EARLY, AND INTERMEDIATE MATURING DROUGHT TOLERANT MAIZE VARIETIES IN GHANA K. Obeng-Antwi, PhD Maize Improvement-CSIR CRI.
Discussion Group: Breeding Jatropha Breeding Break out: What needs to be done? Natural Variation/Analysis of plant genetic resources (need to focus on.
Empowering the “thirsty” plants - ways for drought tolerance In|es Modesto “Plants for Life” International PhD Program – 2016 (course “Plant Biotechnology.
CRISPR-Cas Representing Abundant Potential for Agriculture
By DR. PAUL NJIRUH NTHAKANIO
Agricultural Biotechnology in Turkey
Plant Breeding across times: - challenges and achievements
Moukoumbi, Y. D1. , R. Yunus2, N. Yao3, M. Gedil1, L. Omoigui1 and O
Evaluation of early drought tolerant maize genotypes under low nitrogen conditions Nyasha E. Goredema1, Ms Nakai Goredema2, Ezekia Svotwa1, Gabriel Soropa1,
PHENOTYPING FOR ADAPTATION TO DROUGHT AND LOW-PHOSPHORUS SOILS IN COWPEA (VIGNA UNGUICULATA (L.) WALP.) Nouhoun Belko1, Ousmane Boukar1, Christian.
Biotechnology 101 Wendy Srnic,
Plant Breeding Approach
Mutation-induced variability for improved yield and rust resistance in wheat in hot irrigated environments Abdelbagi M. Ali1, 2, Izzat S.A. Tahir2, Abdalla.
DR. PAUL NJIRUH NTHAKANIO FUNDED / COLLABORATORS
Introduction to Expert Systems
PLANT BREEDING Introduction
RELIABILITY OF POT SCREENING METHOD TO EVALUATE
Parental screening for QTL pyramiding on multiple-abiotic stress
Brown Plant Hopper Resistant Rice in Lower Northern Region of Thailand
Drought Resistance Evaluation of Diverse Wheat Germplasm
WSC remobilization to grain under drought in wheat
Background and Objectives
Plant & Animal Breeding
Results Achieved: Main Deliverables
Backcross Breeding.
(L, C and X) and Full-polarization
Progress in Breeding Orange-fleshed Sweetpotato in sub-Saharan Africa
Brief description of results on genomic selection of CIMMYT maize in Africa (Yoseph Beyene et al.) Several populations each with 200 F2 x tester individuals.
Filled Grains/ Panicle
University of Wisconsin, Madison
ID: 154 Mirza Mofazzal Islam, PhD Chief Scientific Officer and Head
In Vitro Screening.
Presentation transcript:

The Community of Practices ‘‘ Concept Applied to Rice Production in the Mekong Region: Quick Conversion of Popular Rice Varieties with Emphasis on Drought, Salinity and Grain Quality Improvement’’ Cambodian Title: Improvement of Cooking Quality of a Drought Resistant Rice Variety CAR3

Introduction Rice production in Cambodia relies heavily on the rainfed lowland ecosystem, which accounts for 86%. rainfall dramatically affects the rice productivities. Drought can be recognized as a main constraint to increasing in rice production with can cause yield loss up to 50% (Ouk et al., 2001). Although, cooking quality always requite for people since the surpluses of rice production was existed since 1995 (Mak, 1999). on the above the goal is improve rice production, yield with tolerant (abiotic stress), resistant (biotic stress) and fragrance coking quality. Expectation: For increase the rice production in Cambodia with high yield and good cooking quality of drought resistant.

Objective The Cambodian study focus on improving cooking quality in the drought background rice variety using marker assist selection. Materials and methods CAR3 is considered as a drought tolerance variety, used as recipient in the cross. Phkar Rumduol (PRD), is recognize as good cooking quality rice which carry the aromatic scents, medium amylose content, low gelatinization temperature and has long gel consistency (CARDI,2000), used as donor in the scheme and MAS selection are presented in Fig 1

4 lines were selected (57, 61, 68 and 105) Fig.1. Breeding Scheme and MAS selection in improving cooking quality of a drought resistant rice variety CAR3 BC3F1 BC3F2 F1 x CAR3 BC1F1 x CAR3 BC2F1 BC2F2 MAS BC2F4 BC2F3 RF CARDI GCP Nov. 2007 May. 2008 Oct. 2008 May. 2007 BC3F3 CAR3 x PRD May, 2007 MAS, 125 BC2F4 plant, BADH, RM587, RM589 5 lines selected (21, 37, 38, 39 and 103) Nov, 2007 MAS, 94 BC3F1 plants, BADH, RM587, RM589 4 lines were selected (57, 61, 68 and 105) May, 2008 MAS, 484 BC3F2 plants, BADH, RM587, RM589 15 homozygous lines with all markers selected (16, 51, 66, 119, 150, 172, 200, 272, 279, 344, 357, 361, 377 and 442) Sep, 2008 to Feb, 2009 Screening of improvement of cooking quality of drought resistance in CARDI

table 4 : 15 homozygous PRD plants (for all markers) No DNA No Designation   BADH RM587 RM589 1 16 CIR 808-6-2-5- 57-16 2 51 57-51 3 66 57-66 4 119 57-119 5 150 57-150 6 172 CIR 808-6-2-9- 61-20 7 200 CIR 808-6-2-16- 68-27 8 209 68-36 9 272 68-99 10 279 68-106 11 344 68-171 12 357 CIR 808-19-3-3- 105-1 13 361 105-5 14 377 105-21 15 442 105-86

Cooking Quality Test Designation No Aroma GC 1 CIR 808-6-2-5- 57-16 2 84 57-51 67 3 57-66 70 4 57-119 82 5 57-150 86 6 CIR 808-6-2-9- 61-20 81 7 CIR 808-6-2-16- 68-27 85 8 68-36 80 9 68-99 10 68-106 75 11 68-171 76 12 CIR 808-19-3-3- 105-1 72 13 105-5 68 14 105-21 71 15 105-86 73 PRD   83 CAR3 61

Field screening of cooking quality of Drought Resistant, 10 lines Table 5 : Result Data Collected   Mean LSD 5% Prob CV No Trait Irrigated Drained 1 DF 89 12.09 10.36 ** 6.8 5.8 2 PH 90.19 76.85 8.54 11.78 ns 4.7 7.6 3 TN 4.43 3.82 1.60 1.06 18 13.8 4 PL 21.38 20.08 2.68 2.11 6.2 5.2 5 FG 81.54 50.06 32.96 23.83 * 20.1 23.7 6 UG 22.59 31.06 15.23 19.36 33.5 31 7 GW100 2.54 2.25 0.22 0.28 4.3 6.1 8 BM 1.26 0.64 0.45 0.25 17.9 19 9 GY 0.15 0.05697 0.03 0.02 8.6 18.4 10 DTM 119 5.1 11 LD 30.1 12 LR 5.16 3.43 33

Comment Project For this project allow me and other breeders to understand more over our knowledge that we have, such as : the modern breeding program using by MAS, provided the basic vital information for rice breeder to apply in modern breeding program. help the people that have countries bounding in the Mekong River know clearly about the rice problem especially rice production and yield relate to MAS. MAS of rice breeding, is the modern of era, it also expected that the super rice of Cambodia with good cooking quality could be developed in the near future using gene pyramiding concept. We hope so this or other project will be have training activities to build ability, new knowledge for the developing counties to cut down food securities and increase the modern technology.

Work Plan for Future Seed increase 15 homozygous lines at CARDI Field screening drought tolerance again with 15 homozygous lines in 2009 at CARDI. Field evaluation in multi-locations. Cooking quality test.

Conclusion MAS method provides high accuracy and efficiency in detecting traits of interest, Based on the polymorphism of the 3 markers (BADH, RM587 and RM589) used, 10 homozygous lines for PRD were selected The are 5 lines (1, 4, 5, 7, 9) have GC lower then PRD , 2 lines (6, 8) similar and 8 lines (2, 3, 10, 11, 12, 13, 14, and 15) worst. For the aroma have 11 lines (1, 4, 7, 8, 9, 10, 11, 12, 13, 14, & 15) strong aroma like PRD and 4 lines (2, 3, 5, & 6) medium. Base on the screened, - Drained: leaf drying similar CAR3 have 5 lines (2, 6, 7, 8, 10) and Grain yield that best have 4 lines (1, 2, 8, and 9). - Irrigated: best grain yield have 6 lines (1, 2, 5, 6, 7 & 10). After finished this program, we expect that the super rice of Cambodia with good cooking quality of drought resistant could be developed in the near future using gene pyramiding concept.

Acknowledgements On behalf of institute (CARDI), I wish to thanks Dr MEN Sarom and Dr OUK Makara, director of Cambodia Agriculture Research and Development Institute. Dr Apichart Vanavichit, Dr Teerayuth Toojinda and Dr Jonaliza Siangliw , Rice Genes Discovery Unit. That for giving me the opportunity to attend the training program on MAS and Sincerely thanks to all staff of RGDU. Highly appreciate to the Generation Challenge Program (GCP) and the National Center for Genetic Engineering and Biotechnology of Thailand (BIOTEC) for supporting this training program.

Thank You