BAM: Aerobic Plate Count

Slides:

Advertisements

Similar presentations

PHT 381 Lab # 6. Bacterial population count Many bacteriological studies require that we are able to determine the number of m.o per unit volume of a.

Advertisements

ENUMERATION OF MICROORGANISMS

Tap Water Intrusion Effects on Microbial Life Anthony DeRenzo Grade 10 Pittsburgh Central Catholic High School.

Lab 3: Determination of Aerobic colony count in Foods

ASEPTIC TECHNIQUE Removing inoculum from a broth culture

PHT 381 Lab # 6. Bacterial population count Many bacteriological studies require that we are able to determine the number of m.o per unit volume of a.

03 - CONCENTRATION - DILUTIONS CHEMISTRY 30 – UNIT 2 – SOLUBILITY – CH. 16 IN TEXT.

Aerobic Plate Count, Gram Stain, and Isolation

Introduction to Lab Ex. 19: Enumeration of Bacteria

Faculty of Medicine and Health Sciences Microbiology Lab Second semester 2014 prepared by: Mohammad Al-Qadi

Lab 4: Determination of Aerobic colony count in Foods

Bacterial count.

Establishing Standard Food Production Procedures

Aerobic Plate Count, Gram Stain, and Isolation

Growing Microbes L.O: To understand how microorganisms can be safely cultured in a laboratory Answers in your book: Antibiotics do not work on which type.

Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following”

Culturing Yeast Cells on Media. Pre Lab Definitions: Petri Dish: A round, shallow dish used to grow bacteria. Culture: To grow living organisms in a prepared.

Classic Culture Procedures MAJ (First Mi. Last) Chief, Food Analysis Department.

Lab 18 Goals and Objectives: Exercise 21: Enumeration Work as two pairs per group (max 5 groups) Both pairs use same Escherichia coli culture Use pre-poured.

Micro (2-1) TOTAL VIABLE COUNT Dr. Shahzad Ali Assistant Professor Department of Wildlife and Ecology UVAS, Ravi Campus, Pattoki.

Content Standard 1 Select appropriate laboratory glassware, balances, time measuring equipment, and optical instruments to conduct an experiment.

Practical Part Microscopic Examination of Microorganisms Experiments Identification of MOs Different Staining Techniques.

Microbial Count Aim: Count the number of bacterial cells in a provided sample Methods: Total count, Viable count I. Total count This technique involves.

Results of Sources of Contamination. Fungi Bacteria.

Pre Lab Definitions: (Fill these in on your lab paper) Serial: In a series, order or interval. Measured steps. Dilution: Water Down. Pipette: “Little pipe”

T.Monirah Alhammadi.   There are several ways to isolate microbes that produce antibiotics from the soil. Below are some methods used to isolate microbes.

Pure Culture Techniques

Introduction Many studies require the quantitative determination of bacterial populations. The two most widely used methods for determining bacterial.

STANDARD PLATE COUNT PCA or NA growth of wide range of microorganisms not exacting in their nutrition requirements fastidious organisms may not grow colonies.

03 - CONCENTRATION - DILUTIONS CHEMISTRY 30 – UNIT 2 – SOLUBILITY – CH. 16 IN TEXT.

Lab 8Biology You will serially dilute a known mass of soil, make agar plates of the dilutions and estimate the number of microorganisms in the original.

Determination of spectrum of activity of antibiotics.

بسم الله الرحمن الرحيم 140 MICRO LAB 5 : PURIFICATION OF MICROORGANISM.

Do Now  Please write HW in your agenda.  Please update your table of contents.  On the top half of page 52, draw the following images and identify them.

 Many studies require the quantitative determination of bacterial populations. The two most widely used methods for determining bacterial numbers are:

Activity 48 Follow-up Discuss in your groups the difference in results for each neutralization between pairs. Lack of consistency in drop size Error in.

Warm-up 1/25/16 Clear your desk of all materials You will be taking a pop quiz on lab safety, classroom rules and observation vs. inference No Strings.

Total bacterial number is an important indication for food quality

Wednesday 10/14 Day 2 Science Starters Sheet 1. Please have these Items on your desk. Agenda 2- Fill out your Agenda. Video clip Wednesday!

Microbial Growth Growth in Batch Culture

Measurement Labs. Measuring Length Lab Follow the directions to find the length of the following items. 1.Use the meter stick to measure your Science.

Isolation of microorganisms

ENUMERATION OF MICROORGANISM IN FOODS

PHT 381 Lab # 6 Bacterial population count.

Required Practical 6 – Using Aseptic Technique

Bacterial Count.

Chemical and Physical Changes Lab

Sampling Sampling (採樣) 必須使用無菌工具保持低溫樣品保存樣品選擇.

Cat’s Meow Lab.

A quick tutorial in technical writing

Investigations with Membrane Transport

DO NOW VL 0 Monday 8/31 Complete the DO NOW on your handout. Use the sentence stems to complete the sentence. Date DO NOW Answer Your DO NOW should.

The Mendeleev Lab of 1869 Chemistry I Fall 2011.

DO NOW VL 0 Monday 8/31 Complete the DO NOW on your handout. Use the sentence stems to complete the sentence. Date DO NOW Answer Your DO NOW should.

Introduction Many studies require the quantitative determination of bacterial populations. The two most widely used methods for determining bacterial.

Lab 4: Determination of Aerobic colony count in Foods

Exercise 21: Enumeration Work as two pairs per group (max 5 groups)

Social Regard for learning Prepared by: Arielle Jean L. Calub.

Properties of water Pre-Lab.

Organizing Your Laboratory Notebook

Make a pH scale in your notebook.

COMPARATIVE ANALYSIS FLOW-CHART

CULTURE METHODS Culture methods employed depend on the purpose for which they are intended. The indications for culture are: To isolate bacteria in pure.

pure culture isolation

Writing a Lab The basics.

Bundle 3: Matter & Chemical Reactions

DENSITY= Mass/Volume Irregularly shaped objects have no formula for finding the volume. So, to find the volume of a rock, you measure how much water the.

Lab 4: Determination of Aerobic colony count in Foods

Presentation transcript:

BAM: Aerobic Plate Count 1. What does the text say an aerobic plate count (APC) is intended to do?

BAM: Aerobic Plate Count 2. What does the text say regarding what two organizations developed procedures for APC?

BAM: Aerobic Plate Count 3. If you need more procedural details what source would you use?

BAM: Aerobic Plate Count 4. Make a list of the equipment and materials needed for this procedure from Section A. Underline items you do not have at your work station

BAM: Aerobic Plate Count 5.Considering Section A-1 requirements, where in the classroom will you perform these procudures? 6. From section A-1 list three conditions needed for a proper work area

BAM: Aerobic Plate Count 7. From section B, What does the word sterile mean? Why is this critical to the procedure? 8. Why should a fallout pour plate be taken during plating?

BAM: Aerobic Plate Count 9. After reading section B, determine how many petri dishes will be needed for a 10-6 dilution series?

BAM: Aerobic Plate Count 9. After reading section B, determine how many petri dishes will be needed for a 10-6 dilution series? 10. What are other ways to express the term 10-3?

BAM: Aerobic Plate Count 11. If we were to use .5 ml of homogenate in our initial dilution, how much diluet should be used? Use an example from section B to support your answer.

BAM: Aerobic Plate Count 12. What purpose does agar serve?

BAM: Aerobic Plate Count 13. From section B, draw a flow chart of the steps needed to make dilutions down to 10-6. Ending volumes will be 5ml.

BAM: Aerobic Plate Count 14. From section B, draw a flow chart showing how the sample is placed in a petri dish and agar added. Make references to appropriate labs from your lab notebook indicating materials and techniques previously used.