a c b S1. Lee et al., 2011 * RRT-binding PXDLS-binding NAD(H)-binding

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a c b S1. Lee et al., 2011 * RRT-binding PXDLS-binding NAD(H)-binding S422A PXDLS-binding NAD(H)-binding PXDLS-binding K428R G183V Myc/His CtBP1 1 27 121 129 181 204 274 327 352 440 Myc/His Myc/His-CtBP1 FL Myc/His Myc/His-CtBP1/1-140 Myc/His Myc/His-CtBP1/1-369 Myc/His Myc/His-CtBP1/141-440 Myc/His Myc/His-CtBP1/141-300 Myc/His Myc/His-CtBP1/301-440 Myc/His-pcDNA 6Myc-XIAP/351-497 6Myc-XIAP/262-497 6Myc-XIAP/124-497 6Myc-XIAP/FL c 6Myc-XIAP/FL b 6Myc-XIAP/124-497 IP : α-CtBP1 IB : α-Myc * 6Myc-XIAP/262-497 6Myc BIR1 BIR2 BIR3 RING 6Myc-XIAP/FL 1 28 95 164 232 266 331 450 484 497 6Myc BIR2 BIR3 RING 6Myc-XIAP/124-497 6Myc-XIAP/FL 6Myc BIR3 RING 6Myc-XIAP/262-497 6Myc-XIAP/124-497 6Myc RING 6Myc-XIAP/351-497 Lysates 6Myc-XIAP/262-497 6Myc-XIAP/351-497 CtBP1 S1. Lee et al., 2011

a b S2. Lee et al., 2011 DMSO MG132 MG132 HA-Ub Myc/His-pcDNA 6Myc-XIAP 6Myc-cIAP1 - - + - + - + + - + - dCtBP-V5 GST-Ub GFP-Diap1 WT GFP-Diap1 th6 + - + + + - + - + + + - dCtBP-(Ub)n IP : α-V5 IB : α-V5 IP : α-CtBP1 IB : α-HA CtBP1-(Ub)n * dCtBP-V5 6Myc-cIAP1 6Myc-XIAP Lysates Lysates GFP-Diap1 WT/th6 dCtBP-V5 CtBP1 S2. Lee et al., 2011

Supplemental Figure Legends Supplemental Figure 1: (a) Schematic diagram of various CtBP1 domain constructs (b) Schematic diagram of various XIAP deletion constructs (c) Interaction of CtBP1 with various XIAP deletion mutants. HeLa cells were transfected with control or 6Myc-XIAP deletion constructs shown in (S1b). BIR3 is responsible for the interaction with CtBP1. Supplemental Figure 2: (a) Comparison of dCtBP ubiquitination by Diap1 WT or th6 RING mutant (b) In vivo ubiquitination in HeLa cell. 6Myc-XIAP or 6Myc-cIAP1 was co-transfected with HA-Ub. After 24h transfection, cells were treated with DMSO or 20 uM MG132 for 6h.