Interaction of the Lyme Disease Spirochete Borrelia burgdorferi with Brain Parenchyma Elicits Inflammatory Mediators from Glial Cells as Well as Glial.

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Interaction of the Lyme Disease Spirochete Borrelia burgdorferi with Brain Parenchyma Elicits Inflammatory Mediators from Glial Cells as Well as Glial and Neuronal Apoptosis Geeta Ramesh, Juan T. Borda, Jason Dufour, Deepak Kaushal, Ramesh Ramamoorthy, Andrew A. Lackner, Mario T. Philipp The American Journal of Pathology Volume 173, Issue 5, Pages 1415-1427 (November 2008) DOI: 10.2353/ajpath.2008.080483 Copyright © 2008 American Society for Investigative Pathology Terms and Conditions

Figure 1 Visualization of the production of cytokines IL-6 and IL-1β by glial cells in B. burgdorferi-exposed frontal cortex tissue explants and brain sections from animals given stereotaxic inoculations with B. burgdorferi. A: IL-6-producing astrocytes appear pink because of co-localization of IL-6 antibody (labeled blue) with antibody to the astrocyte marker GFAP (red). The spirochetes stained with fluorescein isothiocyanate-labeled B. burgdorferi antibody (Bb) appear green. Unstained tissue appears gray under differential interference contrast (DIC). B: Astrocytes labeled with GFAP (red) in brain tissue slices incubated in medium plus brefeldin A in the absence of spirochetes had no detectable IL-6. C: Visualization of IL-6-producing astrocytes in a tissue section taken at the site of inoculation from an animal given stereotaxic inoculations with live B. burgdorferi. IL-6-producing astrocytes appear yellow because of co-localization of antibody to IL-6 (green) and antibody to the astrocyte marker GFAP (red) in the vicinity of B. burgdorferi antigen stained blue. D: IL-1β-producing microglia appear yellow because of co-localization of antibody to the microglial marker IBA 1 (green) and antibody to IL-1β (red). Spirochetes in this image appear blue. E: Microglia labeled with IBA-1 (green) in brain slices incubated in medium plus brefeldin A in the absence of spirochetes had no detectable IL-1β. The American Journal of Pathology 2008 173, 1415-1427DOI: (10.2353/ajpath.2008.080483) Copyright © 2008 American Society for Investigative Pathology Terms and Conditions

Figure 2 Visualization of the production of IL-8, CXCL13, and COX-2 by glial cells in B. burgdorferi-exposed frontal cortex tissue explants. A: IL-8-producing astrocytes appear yellow because of co-localization of IL-8 antibody (green) and antibody to astrocyte marker GFAP (red). B: IL-8-producing microglia appear yellow because of co-localization of IL-8 antibody (red) and antibody to microglial marker IBA 1 (green). C: Production of CXCL13 in microglia appears yellow because of overlap of antibody to CXCL13 (green) and IBA 1(red). D: COX-2-producing astrocytes are seen as yellow because of overlap of GFAP-staining astrocytes (red) and COX-2 (green). E: COX-2-producing microglia are seen as yellow because of overlap of IBA-1-staining microglia (red) and COX-2 (green). Spirochetes appear blue in all sections. The American Journal of Pathology 2008 173, 1415-1427DOI: (10.2353/ajpath.2008.080483) Copyright © 2008 American Society for Investigative Pathology Terms and Conditions

Figure 3 Oligodendrocyte and neuronal apoptosis in frontal cortex brain tissue exposed to live B. burgdorferi. A: Confocal micrograph showing apoptotic oligodendrocytes as yellow because of co-localization of antibody to active caspase-3 (green) and antibody to S-100 antigen (red). Spirochetes in this section appear blue. B: Percentage of oligodendrocytes (S-100-positive, GFAP-negative) undergoing apoptosis after 4 hours and 8 hours of exposure to live B. burgdorferi as quantified by both the active caspase-3 (AC-3) and in situ TUNEL assays (TUN). C: Confocal micrograph showing apoptotic neurons as yellow because of co-localization of antibody to active caspase-3 (green) and antibody to the neuronal marker NeuN (red). Spirochetes appear blue in color. D: Percentage of neurons undergoing apoptosis after 4 hours and 8 hours of exposure to live B. burgdorferi as quantified by both the active caspase-3 (AC-3) and in situ TUNEL assays (TUN). The American Journal of Pathology 2008 173, 1415-1427DOI: (10.2353/ajpath.2008.080483) Copyright © 2008 American Society for Investigative Pathology Terms and Conditions

Figure 4 Oligodendrocyte apoptosis observed in rhesus CM60 given stereotaxic inoculations with live B. burgdorferi. A: Apoptotic oligodendrocytes show TUNEL-positive nuclei stained green. Oligodendrocytes appear red because of staining with antibody to S-100. B. burgdorferi antigen appears blue in color. B: Percentage of oligodendrocytes (S-100-positive, GFAP-negative) undergoing apoptosis in brain sections from the sites of spirochetal inoculation (A–C) and corresponding control sites (D–F) as quantified by the in situ TUNEL assay. The American Journal of Pathology 2008 173, 1415-1427DOI: (10.2353/ajpath.2008.080483) Copyright © 2008 American Society for Investigative Pathology Terms and Conditions