The Poinsettia 04 Strain A Biotype of Bemisia tabaci New to the Americas T. J. Dennehy Judy Brown.

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Presentation transcript:

The Poinsettia 04 Strain A Biotype of Bemisia tabaci New to the Americas T. J. Dennehy Judy Brown

Outline 1. Evidence that the whitefly collected on poinsettia in Tucson are the Q biotype: a. Frank Byrne, UC-Riverside. b. Judy Brown’s Lab. c. my lab (EARML). 2. Arizona Whitefly Biotypes: 2001. 3. Arizona whitefly biotypes: 2003. Insecticide resistance of the Poinsettia 04 strain. Knack (Pyriproxyfen) Courier (Buprofezin) c. Admire/Provado (Imidacloprid) d. Acetamiprid Thiamethoxam Danitol + Orthene (Fenpropathrin + Acephate)

Extension Arthropod Resistance Management Laboratory Partnership Long-term data collection Long-term industry support Linkages with campus-based research efforts

Testing of Poinsettia 04 Strain by Frank Byrne, UC Riverside, using gell electrophoresis of esterases. The sample we sent Frank contained a mixture of Q and B biotypes.

Results from Judy Brown's Laboratory. C01 sequence homology. The homology tree graphically depicts the close relatedness to Poinsettia 04 strain whiteflies (highlighted) to the Q strains that have been reported in the literature and to the many other strains that Judy's lab has evaluated from around the world.

EARML evaluations of Poinsettia 04 strain confirmed the > 99% homology to the Spain Q type and less than 95% homology to the Arizona B biotype in sequences of the mitochondrial cytochrome oxidase 1 gene.

43 different Arizona whiteflies collected in 2001 from 22 different locations (highlighted). Cultures were reared by EARML and tested jointly by personnel from EARML and Judy Brown's laboratory. All were the B biotype.

Arizona whiteflies collected in 2003 from 29 different locations were all the B biotype B Biotypes Q Biotypes

Knack (pyriproxyfen)

Arizona Whiteflies—B Biotype

Poinsettia 04 strain (Q biotype) vs other Resistant Strains from Arizona

Buprofezin

Courier (buprofezin)

Neonicotinoid Insecticides Active Ingredient Subgroup Producer 1st reg Trade Names Imidacloprid nitroguanidine Bayer 1993 Admire, Confidor, Gaucho, Marathon, Merit, Premise, Provado Acetamiprid cyanoamidines Nippon Soda / DuPont 1996 Mospilan, Assail, Intruder, Tristar Thiamethoxam Syngenta 1997 Actara, Centric, Cruiser, Flagship, Platinum Thiacloprid 2000 Calypso Clothianidin Takeda/Sumitomo / Bayer 2002 Fullswing, clutch, Poncho Dinotefuran Mitsui Starkle Nitenpyram nitromethylene Mitsui/Takeda **** Capstar Nithiazine BASF ***** (Not acting on nicotinic Ach receptor)

Admire/Provado (Imidacloprid)

Centric (acetamiprid)--Cotton

Centric (acetamiprid)--Melons

Centric (acetamiprid)

Acetamiprid vs Thiamethoxam

Actara, Centric, Cruiser, Flagship, Platinum (Thiamethoxam)

Danitol + Orthene