Elastica van Gieson-picro-fuchsin staining The stable connective tissue staining for the differential analysis of nuclei, muscle and elastic fibres

Elastica van Gieson-picro-fuchsin staining An overview staing using 3 different dyes Resorcin-Fuchsin: - elastic fibers Weigert‘s iron hematoxylin: - permanent nuclei stain Van-Gieson‘s picro-fuchsin: - connection tissue

Elastica van Gieson-picro-fuchsin staining Fixaction recomandation: formalin or alcohol infiltration, embedding, cutting, uptake, drying Solvent Time [min] Process xylol, ethanol series, det. water deparaffinize, water resorcin-fuchsin 15:00 elastic stain tap water 01:00 rinse Weigerst‘s iron hematoxylin 05:00 nuclear stain 10:00 wash out hydrochloric acid alcohol 1 % 00:10 differentiate dest. water 00:05 van-Gieson‘s picro-fuchsin solution 02:00 dye (1-5 min) ethanol 96 %, isopropanol, xylol water xylol containing agent cover

Elastica van Gieson-picro-fuchsin staining Dyeing results on standard tissues: nuclei: brown-black connection tissue: red elastic fibres: black musculature: yellow cytoplasma: yellow neuroglia: yellow-orange mucus, amyloid, colloid, hyalin: yellow to red Dyeing results on sponge tissues: nuclei: purple-black background tissue: pink elastic fibres (spongin): purple the stain reacts different on sponge tissue

Elastica van Gieson-picro-fuchsin staining spongin nucleus cyanobacteria spicules

Elastica van Gieson-picro-fuchsin staining

Elastica van Gieson-picro-fuchsin staining

Elastica van Gieson-picro-fuchsin staining