Accucore UHPLC & HPLC Columns Mike Oliver Accucore Product manager July, 2017

What is Accucore?

How Core Enhanced technology Delivers: Better Separations Accucore UHPLC & HPLC columns are based on solid core particles which deliver fast and highly efficient separations. Key Components

Why Core Enhanced technology works: Better Separations The factors that affect chromatographic efficiency are resistance to mass transfer, longitudinal diffusion and eddy diffusion, the A, B and C terms respectively from the van Deemter equation. Uniform packed bed Reduced void volume Fast mass transfer Results in Better Separations

Accucore HPLC Columns – Features and Benefits Ultimate Core Performance Maximizing Your Investment Fast Separations Increase sample throughput and decrease solvent costs High Peak Capacity Separate and identify more peaks Increased Sensitivity Detect trace amounts Lower Pressure Use existing HPLC instruments or prolong the life of UHPLC instruments Loading Capacity Analyze a wide range of concentrations Reproducible Chromatography Have confidence in your separations Long Lifetime Use columns for longer Wide Selectivity Use the right column for your analysis

Accucore UHPLC & HPLC column family Fdghhd yjtyjtjk

Accucore Vanquish UHPLC Columns

What is Accucore Vanquish? Next generation UHPLC column Founded on state-of-the-art Core Enhanced Technology 1.5 µm solid core particles Designed in synergy with the high end UHPLC systems to deliver: Better Separations: high efficiency enables the separation of very complex mixtures More Results: High efficiency at high flow rates Easier Interactions: Seamless workflow solution, for simple and easy separations

Core Enhanced Technology Delivers: Better Separations More efficient at optimum flow than fully porous sub 2 micron More efficient at optimum flow than 2.6 micron solid core Wider “no-efficiency-loss” range than any other column technology Effectively, as solution with Vanquish delivering unmatched resolution at high flows => pure productivity!

Why 1.5um provides: Better Separations Efficiency = Better Separations 0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 3.00 3.25 3.50 3.75 4.00 4.25 4.50 4.75 5.00 5.25 5.50 5.75 6.00 6.25 6.50 6.75 7.00 min 3 2 1 Accucore Vanquish 1.5 m, 295k N/m Accucore 2.6 m, 196k N/m Fully porous 1.7 m, 176k N/m 40% efficiency gain

Why Core Enhanced technology Delivers: More Results Accucore Vanquish C18 1.5 µm 100 x 2.1mm Solid Core C18 2.6 µm 100 x 2.1mm The higher efficiency provided by the Accucore Vanquish column allows for shorter run times as we can increase the flow rate and at the same time provide better separations. In this example we can see we have a 40% time saving on the run time for the separation of 18 pesticides at a flow rate of 650 compared to 380ul/min compared to the Accucore 2.6um column. Average increase in resolution of 24% Average increase in peak capacity of 220% 40% time saving and Better Separations

Why Core Enhanced technology Delivers: More Results Accucore Vanquish v’s 1.6µm competitor solid core column Accucore Vanquish C18 1.5 µm 100 x 2.1mm C18 1.6 µm 100 x 2.1mm Average increase in resolution of 6% Average increase in peak capacity of 11%

Why Core Enhanced technology Delivers: More Results Accucore Vanquish v’s 1.7µm competitor solid core column Accucore Vanquish C18 1.5 µm 100 x 2.1mm C18 1.7 µm 100 x 2.1mm Average increase in resolution of 17% Average increase in peak capacity of 22%

Accucore Vanquish Delivers: More Results Rapid Screening of 47 Drugs of Abuse in <4min Overlaid selected-reaction monitoring chromatograms showing detection of 47 compounds

Accucore Vanquish Delivers: More Results Separation of isobaric compounds Chromatographic resolution of N-desmethyltapentadol and MDEA with out common precursor and product ions Chromatographic resolution of codeine and hydrocodiene with common precursor (300 u) and product ions Chromatographic resolution of naltrexone and 6-acetylcodine with common precursor (342 u) and product ions

Accucore Vanquish Delivers: More Results Rapid Screening of 36 Veterinary Antibiotics in <5min Overlaid selected-reaction monitoring chromatograms showing detection of 36 compounds

Why Core Enhanced technology Delivers: More Results Reproducibility The advanced bonding and automated packing technology used in the manufacture of Accucore Vanquish UHPLC columns results in exceptionally reproducible chromatography. 4000 injections @ 500 bar

Extended capabilities - Vanquish UHPLC columns Next generation UHPLC columns delivering: Better Separations: high efficiency enables separation of very complex mixtures More Results: High efficiency at high flow rates Easier Interactions: Seamless workflow solution, for simple and easy separations Wider Capabilities: Wide range of column selectivity's and formats

Accucore UHPLC & HPLC Columns

Accucore HPLC Column Family

Accucore HPLC Column Family Thermo Scientific™ Accucore™ HPLC columns provide HPLC and UHPLC users with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub-2µm particles Thermo Scientific™ Accucore™ columns for biomolecules provide nanoLC, HPLC and UHPLC users separating biomolecules with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub- 2µm particles Thermo Scientific™ Accucore™ XL HPLC columns allow users of conventional HPLC methods to enjoy increased peak resolution and lower limits of detection through much higher efficiencies than those offered by columns packed with 5 μm, 4 μm or even 3 μm fully porous particles

Accucore – Product Range Formats Analytical columns, Defender guard cartridges Phases Accucore RP-MS Optimized for MS detection, excellent combination of speed and quality of separation Accucore C18 Optimum retention for non-polar analytes Accucore C8 Lower hydrophobicity than C18 recommended for analytes with moderate hydrophobicity Accucore aQ Compatible with 100% aqueous mobile phases, special selectivity for polar analytes Accucore Polar Premium Rugged amide embedded C18 phase that offers complementary selectivity to C18 Accucore Phenyl-Hexyl Unique selectivity for aromatic and moderately polar analytes Accucore Phenyl-X Unique reversed-phase shape selectivity with high aromatic selectivity Accucore PFP Alternative selectivity to C18, particularly for halogenated analytes Accucore C30 High shape selectivity for hydrophobic, long chain, structurally related isomers Accucore HILIC Enhanced Retention of polar and hydrophilic analytes Accucore Urea-HILIC Unique HILIC selectivity and low ion exchange activity

Accucore XL – Product Range Formats Analytical columns, Drop-in guard cartridges Phases Accucore XL C18 Compatible with conventional HPLC methods providing optimum retention for non-polar analytes Accucore XL C8 Lower hydrophobicity than C18 recommended for analytes with moderate hydrophobicity and compatible with conventional HPLC methods

Accucore HPLC Columns High-pressure hardware 600 bar pressure rating Etched end fittings Purple Rings 4 lengths 30mm 50mm 100mm 150mm 3 IDs 2.1mm 3.0mm 4.6mm

Accucore Defender TM Guard Cartridges Designed specifically to work with high speed, high efficiency separations Compatible with UNIGUARD holders Pack of 4 cartridges 1 length 10mm 3 IDs 2.1mm 3.0mm 4.6mm

Accucore – Speed Improve throughput and save costs Minutes 1 2 3 4 5 6 7 8 9 10 -100 Accucore RP-MS 2.6m,100 x 2.1 mm ΔP = 218 bar Rs = 2.50 Fully porous C18 5m, 150 x 4.6 mm ΔP = 59 bar Rs = 2.64 Method Transfer Calculator: www.thermoscientific.com/crc Fully porous 5 μm 150 x 4.6 mm 35 – 60 %B in 10.0 min 17 min run time 1000 L/min solvent used 17 mL Accucore RP-MS 2.6 μm, 100 x 2.1 mm 35 – 60 %B in 3.5 min 6 min run time 400 L/min solvent used 2.4 mL Accucore RP-MS 2.6 μm, 50 x 2.1 mm 35 – 60 %B in 1.8 min 3 min run time 400 L/min solvent used 1.2 mL Accucore RP-MS 2.6m, 50 x 2.1 mm ΔP = 120 bar Rs = 1.51 Improve throughput and save costs

Accucore - Same System, Same Method, Better Results Column P (bar) N (plates) Peak 2 S/N FPP 5 µm 239 10538 534 XL 4 µm 312 18274 1202 Fully Porous C18, 5m Columns: 150 x 4.6 mm Mobile phase: 66.3:33.7 (v/v) water with phosphoric acid, pH 2.5:methanol Flow rate: 2 mL/min Column temperature: 30 °C Detection: UV at 214 nm Injection volume: 5 µL Valerophenone Ibuprofen Accucore XL C18, 4 m 73% higher efficiency 125% higher sensitivity

Accucore – Ruggedness and Reliability Accucore XL C8 4.0 μm 50 x 2.1 mm Injection 2200 Injection 1500 Injection 750 Injection 1 Separation of naproxen, fenoprofen and ibuprofen, with theophylline as a t0 marker Rely on columns and get extended use from them

Accucore BioLC Columns

Accucore HPLC Column Family

Accucore HPLC Column Family Thermo Scientific™ Accucore™ HPLC columns provide HPLC and UHPLC users with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub-2µm particles Thermo Scientific™ Accucore™ columns for biomolecules provide nanoLC, HPLC and UHPLC users separating biomolecules with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub- 2µm particles Thermo Scientific™ Accucore™ XL HPLC columns allow users of conventional HPLC methods to enjoy increased peak resolution and lower limits of detection through much higher efficiencies than those offered by columns packed with 5 μm, 4 μm or even 3 μm fully porous particles

Accucore Bio – Product Range Formats Analytical columns, Defender guard cartridges, nanoViper columns Phases Accucore 150-C18 Phase designed for the separation of peptides Accucore 150-C4 Lower hydrophobicity for optimal retention of proteins and larger peptides Accucore 150-Amide-HILIC Designed for the separation of hydrophilic biomolecules in HILIC mode. An excellent choice for glycan separations

Separate peaks of interest Accucore – Resolution Accucore 150-C18 Peak no. Peptide 1 GY 2 VYV 3 Met-Enkephalin 4 Angiotensin III 5 Leu-Enkephalin 6 Ribonuclease A 7 Insulin Conventional Solid Core C18 < 2 µm Wide Pore Fully Porous C18 Separate peaks of interest

Proteins – Excellent Resolution vs < 2 µm Wide Pore Accucore 150-C4 Backpressure: 185 bar Sharper and higher peaks than < 2 µm Wide Pore Fully Porous C4 Better resolution and sensitivity Significantly lower backpressure < 2 µm Wide Pore Fully Porous C4 Backpressure: 320 bar

Peptides – High Peak Capacity – greater sensitivity Higher peak capacity than Conventional solid core C18 < 2 µm wide pore fully porous C18 Higher peak capacities facilitate increased peptide identifications

Peptides – Reproducible Separations Peak # Name tR % RSD 7 Insulin 13.58 0.11 Precision of retention times is critical for reliable analysis The Accucore 150-C18 column exhibits excellent retention time reproducibility

Proteins – Reproducible Results tR % RSD PWHH As Ovalbumin (45 kDa) 9.30 0.06 0.05 1.14 Precision of retention times is critical for reliable analysis The Accucore 150-C4 column exhibits excellent retention time reproducibility

Proteins – Proven Loading Capacity As the load on the Accucore 150-C4 column increases up to 1200 pmoles peak shapes remain very sharp

Positioning

Market Requirements Omics Bioanalysis Clinical Environmental Complexity of sample Bioanalysis Clinical Environmental HTS/Library QA/QC Speed of Analysis

Product Positioning Accucore BioLC Accucore Vanquish UHPLC Complexity of sample Accucore 2.6µm UHPLC/HPLC Accucore 4µm HPLC Speed of Analysis

Supporting collateral?

Marketing Plans www.thermofisher.com/accucore Technical Guide Phase guide Application notes Webinars Commercial web based training One minute highlight Videos Social media Landing page Product page Method conversion tool Advertorials www.thermofisher.com/accucore www. appslab.thermofisher.com

Accucore Column Family Summary Accucore UHPLC & HPLC Columns Accucore Columns for Biomolecules Accucore XL HPLC Columns Ultimate Core Performance - Speed and Selectivity Combined Solid core particles for fast separations with superb resolution Wide range of phases to meet all your separation needs Exceptionally rugged and easy to use columns