Cancer Center Building Rm. 401

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Presentation transcript:

Cancer Center Building Rm. 401 Molecular and Cellular Immunology Core, Health Sciences Microscopy and Imaging Core, & BD Biosciences Present Multicolor Flow Cytometry Seminar: Implications Of Relative Fluorochrome Brightness In Resolving Your Target Populations & The Significance Of Antigen Density In Choosing The Appropriate Antibody/Color Combination & The Power of INDEX Sorting In Single Cell Research The Past 40 Years Has Seen Amazing Advancements In Flow Cytometry Technology, As We Are Seeing Instruments Capable Of 18-Color Analysis Become Standard. However, Multicolor Panel Design Until Now Has Been Optimized Largely By Trial And Error. Based On Our Current Findings With The Newest Fluorochrome Technology, We Will Share An Approach To Panel Design That Enables Scientists To Unlock The Full Potential Of Their Research Offered By Innovations In Flow Cytometry Systems. The MCI Core's BD FACSAria Sorter Just Underwent An Upgrade: We Are Now Able To Execute INDEX Sorting. INDEX Sorting Is The Ability To Sort Single Cells Into 96 well/PCR Plates And Allowing One To Record The Fluorescence Levels Of The One Sorted Cell, And Tie That Data With Any Downstream Application. We Will Explain The Capabilities And Possibilities Of Such A Powerful Tool In Single Cell Research. Lisa Bellemare, PhD Technical Applications Specialist, BD Biosciences Thursday September 15, 2016 10am Cancer Center Building Rm. 401 FOR FURTHER INFO PLEASE CONTACT ALEXANDRA GURARY (692-1794 OR gurary@hawaii.edu)