How is life as we know it powered? Big Idea/Question…. How is life as we know it powered? PS CR

Unit 4 Part 5 Cellular Respiration: Inputs, Outputs & mitochondria

Learning Targets 5. Identify the inputs and outputs and location of glycolysis, Krebs cycle, and oxidative phosphorylation. 7. Compare and contrast the structure and function of mitochondria and chloroplasts.

Cellular Respiration Location?

Mitochondria Be able to identify: Inner membrane Outer membrane Cristae Matrix

Cellular Respiration What is it? Who does it? How?..... Converts potential chemical (glucose) energy into ATP 1 glucose can produce somewhere around 34 ATP molecules!! Who does it? Plants, animals and some bacteria How?.....

Cellular respiration The process: Reactants (inputs): Glucose & Oxygen Products (Outputs): CO2, H2O & ATP

Learning Check What are some similarities between mitochondria and chloroplasts? Where does Cellular respiration occur? What are the inputs for CR? What are the outputs for CR?

Unit 4 Cell Energetics Part 6 Glycolysis, Bridging Process, the Krebs Cycle/Citric Acid Cycle & Oxidative phosphorylation

Learning Targets 5. Identify the inputs and outputs and location of glycolysis, Krebs cycle, and oxidative phosphorylation.

Electron Carriers in CR NAD+  NADH FAD+  FADH2

Cellular Respiration Glycolysis Bridging Process Krebs Cycle Electron Transport Chain

Glycolysis Glycolysis  splitting glucose Location  cytoplasm Anaerobic (no O2 required) Utilized by ALL cells Steps Energy Investment Energy Payoff Per Glucose Produces 2 ATP ( 5 % of available energy) 2 NADH 2 Pyruvate

Glycolysis Animation http://highered.mcgraw-hill.com/sites/0072507470/student_view0/chapter25/animation__how_glycolysis_works.html

Now what??

Bridging (Transition) Process Aerobic  requires O2 as final electron acceptor (happens in ETC) Location --inside mitochondria “One-Two Punch” Carbonyl group released as CO2 NAD+ reduced to NADH Leaves Acetyl--picked up by CoA & becomes Acetyl CoA Surplus of ATP  acetyl-CoA gets stored as lipid Little ATP  acetyl-CoA enters Krebs cycle & makes ATP

Krebs Cycle aka Citric Acid Cycle Location--Matrix of mitochondria Aerobic  requires O2 as final e- acceptor Produces: CO2 (from carbonyl) NADH FADH2 ATP

Electron Transport Chain Location--Membrane of cristae Aerobic  requires O2 (final electron acceptor) Follow the electrons—and protons! Animation vcell.ndsu.nodak.edu/animations/etc/first.htm

Oxidative Phosphorylation Electrons power proton pumps (which are imbedded proteins)  pump H+ into intermembrane space (ETC) Generates ATP when H+ diffuse back into matrix thru ATP Synthase (Chemiosmosis) NAD+ powers 3 pumps  3 ATP FAD+ powers 2 pumps  2 ATP http://www.nature.com/nrg/journal/v2/n5/animation/nrg0501_342a_swf_MEDIA1.html

Oxidative Phosphorylation e- transferred to oxygen through proton pumps  builds up proton gradient Generates ATP through ATP Synthase

Learning Checks What are the 4 parts of Cellular Respiration, and where are they located? Mitochondria transfer _____________ energy from ________ to ATP; chloroplasts transform ___________energy into the chemical energy.