INSTITUTE FOR TROPICAL BIOLOGY AND CONSERVATION Phytochemical Analysis and Biological Potentials of Bornean Lindera polyantha (Bl.) Boerl. Julius Kulip*, Takashi Kamada and Charles S. Vairappan Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia. Introduction Developed in Hex: EtOAc (3: 1) Compound 1 against four strains of human pathogenic bacteria Documentation of traditional knowledge on medicinal plants was carried out all around the world. The endeavours is to continue the poor transmission of traditional knowledge to younger generations, this knowledge is important in the exploitation of plants pharmaceutical industries, and the need to conserve rare or highly demanded plant species in traditional medicine. Traditional medicinal plants documentations and research were started in early 1980s in Sabah. The medicinal herb, Lindera polyantha (Bl.) Boerl. is listed one of the 1,300 medicinal herbs by Kulip in 2004. However, there is no research for chemical analysis against Lindera polyantha (Bl.) Boerl. Hence, we report the phytochemical analysis as well as bioactivities of isolated pure compounds from this plant and create healthy drink using traditional knowledge in Sabah. Escherichia coli Salmonella enteritidis Vibrio cholerae Salmonella thypi Molybdophos- phoric acid 366 nm HPTLC Profiling Antibacterial Bioassay The ethanolic extract yielded three pure compounds by chromatographic techniques. These major secondary metabolites such as 5-hydroxy-2-(10-hydroxy-40-oxo-cyclohex-20-en-10-yl)-6,7-dimethoxy-2,3-dihydro-4H-chromen-4-one (1), linderone (2) and methyllinderone (3) were elucidated based on NMR spectroscopic analysis. Besides, these compounds reported various biological activities such as anti-inflammation, anti-fungal, anti-oxidant and anti-cancer which suggested that these compounds could be used as lead pharmaceutical agents. INSTITUTE FOR TROPICAL BIOLOGY AND CONSERVATION Voucher specimen of Lindera polyantha (Bl.) Boerl. 1 Sampling Location Lindera spp. are distributed from temperature regions to Southeast Asia but are mainly distributed in tropical and subtropical regions. The genus has around 100 species. The Chinese traditionally used to treat stomach, urinary system diseases and rheumatic pain with this plant. 2 3 Secondary metabolites from Lindera polyantha (Bl.) Boerl. Commercial Potentialities Results and Discussion Finally, our ongoing effort in collaboration with the company, Pemborong Mangkab Enterprise (PME) to create new traditional medicinal healthy drink has provided a new market for investors and researches to enjoy the fruitfulness of sustainable development. 1. Identification of the plant Lindera polyantha (Bl.) Boerl. or local name as Roiros (Dusun Tuaran, Sabah) was an endemic evergreen tree grows in Borneo Island. It has fragrance and was a traditional local medicine used for treating several symptoms. According to the ethnobotanical studies by Kulip in 1996, it was used as anti itchy, blood cleansing and to get rid of gas in Dusun community since time immemorial. Novelty and Inventiveness 1. Three bioactive secondary metabolites were isolated. 2. Published two international and one domestic journals. 2. Extraction, Isolation and Structure Elucidation Lindera polyantha (Tuaran) Structure elucidation (1D and 2D NMR, HR-MS) Publications Kulip, J., Kamada, T. and Vairappan C.V. 2015. Natural Products Chemistry and Research, Vol. 3 Issue 4, 184-185. Kulip, J., Kamada, T. and Vairappan C.V. 2016. Aromatic compounds from Lindera polyantha (Bl.) Boerl. (Lauraceae), a Bornean Medicinal Herb. Natural Product Communications, submitted. Kulip, J. 2015. Trends of Ethno-Ecosystem Services in Two Dusun Villages in Sabah, Malaysia. Proceedings of the Seminar on Documenting Traditional Forest-Related Knowledge for Ecosystem Services in ASEAN Countries. Organized by Asia Pacific Association for Forestry Research Institute (APAFRI) and Asian Center for Traditional Forest Knowledge (ACTFOK), Korea Forest Research Institute (KFRI). PP: 135-182. Kulip, J. (2004). Medicinal plants of Sabah: How much do we know?. Jointly organised by FRIM, UMS and Borneo Herbal Cooperation (SaProceedings of the National Seminar on Medicinal Plants and the Herbal Industry of Malaysia: Opportunities and Challengesbah S/B/ (BHCS). Kulip, J. (1996). Medicinal and other useful plants of the Dusun/Kadazan people in Tambunan, Sabah, Malaysia. Proceedings of the 4th Biennial International Meeting Conference, University of Brunei Darussalam, Bruneri Darussalam. Extraction (EtOH soaked) Screening of selected bioassay such as anti-bacteria. Purification (Column chromatography, preparative TLC and HPLC) Acknowledgement This work was supported financially by the SGPUMS Research Grant SLB0108-2015. The authors would like to acknowledge the assistance of Mr. Johnween Galuk from Kg. Tudan, Tuaran for supplying plant materials.