SP receptor neurokinin 1 receptor (NK1R) is expressed in adipocytes and preadipocytes, and SP interferes with differentiation. 3T3-L1 preadipocytes were.

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Presentation transcript:

SP receptor neurokinin 1 receptor (NK1R) is expressed in adipocytes and preadipocytes, and SP interferes with differentiation. 3T3-L1 preadipocytes were differentiated with/without SP supplementation (10 nM) in the differentiation medium and evaluated by mi... SP receptor neurokinin 1 receptor (NK1R) is expressed in adipocytes and preadipocytes, and SP interferes with differentiation. 3T3-L1 preadipocytes were differentiated with/without SP supplementation (10 nM) in the differentiation medium and evaluated by microscopy at days 0, 2, 4, 6, and 8. Representative micrographs are shown. Inset: RNA from 3T3-L1 preadipocytes and mature adipocytes was extracted, and gene expression of NK1R was evaluated by real-time PCR. Gene expression is presented relative to expression of the housekeeping gene GAPDH for an average of 3 separate determinations. Pierre Miegueu et al. Am J Physiol Gastrointest Liver Physiol 2013;304:G420-G427 ©2013 by American Physiological Society