Figure 1. Genomic instability biomarkers in non-syndromic cleft lip and/or palate patients and control children. Frequency of MN (a), nucleoplasmic bridges.

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Figure 1. Genomic instability biomarkers in non-syndromic cleft lip and/or palate patients and control children. Frequency of MN (a), nucleoplasmic bridges (b) and nuclear buds (c) of clefts and control groups. Arrows point to a micronuclei (d), a nucleopasmic bridge (e) and a nuclear bud (f). Photos captured with 400× magnification. From: Analysis of genome instability biomarkers in children with non-syndromic orofacial clefts Mutagenesis. 2017;32(2):313-321. doi:10.1093/mutage/gew068 Mutagenesis | © The Author 2017. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Figure 3. Transcriptogram from non-syndromic cleft lip/palate (NSCL/P) patients. Expression NSCL/P patient biopsies (n = 7) and normal individual biopsies (n = 6) were obtained from Gene Expression Omnibus, GSE42589. The transcriptogram is shown in the centre. Gene position is shown on the x-axis and relative expression is shown on the y-axis. The dotted line represents the normalised control expression average. The solid black line represents the NSCL/P expression average relative to control. Red marks highlight the different expressed peaks (FDR < 0.05). Solid bars represent cluster size and are coloured according to their respective PPI network, shown on the periphery. Networks were functionally characterised by Gene Ontology Biological Process enrichment, as described in the methods section. Values in parenthesis represent the rate of cluster genes belonging to the respective GO term. From: Analysis of genome instability biomarkers in children with non-syndromic orofacial clefts Mutagenesis. 2017;32(2):313-321. doi:10.1093/mutage/gew068 Mutagenesis | © The Author 2017. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Figure 2. New nuclear morphologies observed in binucleated cells from CBMN assay. Lymphocytes with a circular (a) and horse-shoe (b) nuclei from blood samples of non-syndromic cleft lip/ palate children. Photos captured with 400× magnification. From: Analysis of genome instability biomarkers in children with non-syndromic orofacial clefts Mutagenesis. 2017;32(2):313-321. doi:10.1093/mutage/gew068 Mutagenesis | © The Author 2017. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.