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Supporting Information

Figure S1. Calibration curve for the stable isotope labeled peptides CYP1A2: YLPNPALQR* y = 2.2e+004 x + 886 (r = 0.9972) 10 20 30 40 50 Concentration (pg/μL) 0.0 2.0e5 4.0e5 6.0e5 8.0e5 1.0e6 Peak Area Intensity Figure S1. Calibration curve for the stable isotope labeled peptides derived from P450 isoforms

Figure S1. Calibration curve for the stable isotope labeled peptides CYP2B6: FSVTTMR* y = 1.84e+003 x + 284 (r = 0.9951) 10 20 30 40 50 0.0 2.0e4 4.0e4 6.0e4 8.0e4 1.0e5 Peak Area Intensity Concentration (pg/μL) Figure S1. Calibration curve for the stable isotope labeled peptides derived from P450 isoforms.

Figure S1. Calibration curve for the stable isotope labeled peptides CYP3A4: EVTNFLR* y = 3.54e+003 x + 165 (r = 0.9962) 3.0e5 2.4e5 Peak Area Intensity 1.8e5 1.2e5 6.0e4 0.0 20 40 60 80 100 Concentration (pg/μL) Figure S1. Calibration curve for the stable isotope labeled peptides derived from P450 isoform.

Figure S1. Calibration curve for the stable isotope labeled peptides CYP3A5: SLGPVGFMK* y = 1.42e+003 x + 205 (r = 0.9958) 20 40 60 80 100 0.0 3.0e4 6.0e4 9.0e4 1.2e5 1.5e5 Peak Area Intensity Concentration (pg/μL) Figure S1. Calibration curve for the stable isotope labeled peptides derived from P450 isoforms.

Figure S1. Calibration curve for the stable isotope labeled peptides CYP2C19: GHFPLAER* y = 2.26e+004x + 1.04e+003 (r = 0.9953) 10 20 30 40 50 0.0 2.0e5 4.0e5 6.0e5 8.0e5 1.0e6 Peak Area Intensity Concentration (pg/μL) Figure S1. Calibration curve for the stable isotope labeled peptides derived from P450 isoform.

Figure S1. Calibration curve for the stable isotope labeled peptides CYP2C9: SHMPYTDAVVHEVQR* y = 3.42e+004x +- 1.31e+003 (r = 0.9981) 20 40 60 80 100 0.0 6.6e5 8.0e5 9.4e5 1.8e6 3.2e6 Peak Area Intensity Concentration (pg/μL) Figure S1. Calibration curve for the stable isotope labeled peptides derived from P450 isoform.

Figure S1. Calibration curve for the stable isotope labeled peptides CYP2E1: GIIFNNGPTWK* y = 2.39e+003x +128(r = 0.9957) 10 20 30 40 50 0.0 3.0e4 4.8e4 6.6e4 8.4e4 1.2e5 Peak Area Intensity Concentration (pg/μL) Figure S1. Calibration curve for the stable isotope labeled peptides derived from P450 isoform.

Figure S2. Triplicate analysis of the human liver microsome samples with the developed method.

Table S1. Concentration and RSD of the P450 isoforms by triplicate analysis of the human liver microsome samples. Sample1 Sample2 Sample3 Average Value RSD (%) CYP1A2 32.274 31.737 30.867 31.626 2.244 CYP2B6 2.192 2.252 2.090 2.178 3.746 CYP3A4 38.913 41.484 42.931 41.109 4.950 CYP3A5 6.048 5.518 5.761 5.776 4.593 CYP2C9 9.017 9.163 9.545 9.242 2.946 CYP2C19 0.976 1.099 1.053 1.043 5.992 CYP2E1 9.580 9.594 9.444 9.540 0.871