The largest outbreak of hand; foot and mouth disease in Singapore in 2008: The role of enterovirus 71 and coxsackievirus A strains Yan Wu, Andrea Yeo,

Slides:

Advertisements

Similar presentations

Epidemiological trends and the effect of airport fever screening on prevention of domestic dengue fever outbreaks in Taiwan, 1998–2007 Mei-Mei Kuan, Ting.

Advertisements

Development of a foundation for a case definition of post-treatment Lyme disease syndrome John N. Aucott, Lauren A. Crowder, Kathleen B. Kortte International.

Robert Koch and the ‘golden age’ of bacteriology Steve M. Blevins, Michael S. Bronze International Journal of Infectious Diseases Volume 14, Issue 9, Pages.

Syndromic classification of rickettsioses: an approach for clinical practice Álvaro A. Faccini-Martínez, Lara García-Álvarez, Marylin Hidalgo, José A.

Early impact of pneumococcal conjugate vaccine on invasive pneumococcal disease in Singapore children, 2005 through 2010 Koh Cheng Thoon, Chia Yin Chong,

The potential of social networks for early warning nad outbreak detection systems: the swine flu Twitter study P. Kostkova, E. de Quincey, G. Jawaheer

Yang Luo, Guo-Ping He, Jijan-Wei Zhou, Ying Luo

T. Sternfeld, V. Spöri-Byrtus, C. Riediger, R. Langer, H. Friess, R. M

Intrapopulation variability in mutator prevalence among urinary tract infection isolates of Escherichia coli A. Couce, N. Alonso-Rodriguez, C. Costas,

Adjunctive therapy with interferon-gamma for the treatment of pulmonary tuberculosis: a systematic review X.-F. Gao, Z.-W. Yang, J. Li International.

Antonio Cascio, Chiara Iaria, Paolo Ruggeri, Walter Fries

Neonatal tetanus elimination in Pakistan: progress and challenges

Multi-drug carbapenem-resistant Klebsiella pneumoniae infection carrying the OXA-48 gene and showing variations in outer membrane protein 36 causing an.

Effect of climate variables on dengue incidence in a tropical Caribbean municipality of Colombia, Cerete, 2003–2008 Salim Mattar, Victor Morales, Alexander.

Odontogenic maxillofacial space infections at a tertiary care center in North India: a five- year retrospective study George C. Mathew, Laxman Kumar Ranganathan,

The accuracy and validity of a weekly point-prevalence survey for evaluating the trend of hospital-acquired infections in a university hospital in Turkey

Comparison of two strategies to prevent varicella outbreaks in housing facilities for asylum seekers Serge de Valliere, Naim Cani, Maya Grossenbacher,

P.-Y. Lévy Clinical Microbiology and Infection

Isolation of Kingella kingae in the oropharynx during K

Accurate genotyping of hepatitis C virus through nucleotide sequencing and identification of new HCV subtypes in China population Y.-Q. Tong, B. Liu,

Outbreak of brainstem encephalitis associated with enterovirus-A71 in Catalonia, Spain (2016): a clinical observational study in a children's reference.

Detection of Anaplasma phagocytophilum and Ehrlichia sp

Fast and low-cost decentralized surveillance of transmission of tuberculosis based on strain-specific PCRs tailored from whole genome sequencing data:

Genetic detection of Dobrava/Belgrade virus in a Czech patient with Haemorrhagic fever with renal syndrome A. Papa, H. Zelená, D. Barnetová, L. Petroušová

E. Descloux, C. La Fuentez, Y. Roca, X. De Lamballerie

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Clinical Microbiology and Infection

Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR T. Kallonen, J.

Development of a real-time fluorescence resonance energy transfer PCR to identify the main pathogenic Campylobacter spp. A. Ménard, F. Dachet, V. Prouzet-Mauleon,

J. Lu, H. Zeng, H. Zheng, L. Yi, X. Guo, L. Liu, L. Sun, X. Tan, H

West Nile virus outbreak in Israel in 2015: phylogenetic and geographic characterization in humans and mosquitoes Y. Lustig, Z. Kaufman, B. Mannasse,

Successful control of a large outbreak of HIV infection associated with injection of cathinone derivatives in Tel Aviv, Israel E. Katchman, R. Ben-Ami,

Accurate genotyping of hepatitis C virus through nucleotide sequencing and identification of new HCV subtypes in China population Y.-Q. Tong, B. Liu,

L. Dubourg Clinical Microbiology and Infection

Comprehensive analysis of mutations in the hepatitis delta virus genome based on full- length sequencing in a nationwide cohort study and evolutionary.

Development and Clinical Implementation of a Combination Deletion PCR and Multiplex Ligation-Dependent Probe Amplification Assay for Detecting Deletions.

A. Papa, K. Dumaidi, F. Franzidou, A. Antoniadis

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Unexpectedly high prevalence of Treponema pallidum infection in the oral cavity of human immunodeficiency virus-infected patients with early syphilis.

Olivier Gruselle, Thierry Coche, Jamila Louahed

Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay P. Jokela,

Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China J. Sun, M. Zhou, Q. Wu, Y.

F. Magurano Clinical Microbiology and Infection

Hepatitis A outbreaks The Lancet Infectious Diseases

Enterovirus D68 nosocomial outbreak in elderly people, France, 2014

A novel phlebovirus in Albanian sandflies

A. Papa, K. Xanthopoulou, S. Gewehr, S. Mourelatos

Detection of Wolbachia genes in a patient with non-Hodgkin's lymphoma

W. Ibrahim, N. Boukhadra, D. Nasri-Zoghlami, P. Berthelot, S. Omar, T

The significance of rhinovirus detection in hospitalized children: clinical, epidemiological and virological features J.C. Rahamat-Langendoen, A. Riezebos-Brilman,

Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective.

Emergence of Crimean–Congo haemorrhagic fever in Greece

Screening and detection of human enterovirus 71 infection by a real-time RT-PCR assay in Marseille, France, 2009–2011 C.Y.Q. Tan, G. Gonfrier, L. Ninove,

Intrapopulation variability in mutator prevalence among urinary tract infection isolates of Escherichia coli A. Couce, N. Alonso-Rodriguez, C. Costas,

Detection of IgA and low-avidity IgG antibodies for the diagnosis of recent active toxoplasmosis José Gutiérrez, María Rodríguez, Gonzalo Piédrola, María.

Human infection with novel G3P[25] rotavirus strain in Taiwan

Molecular epidemiology of enterovirus 71, coxsackievirus A16 and A6 associated with hand, foot and mouth disease in Spain M. Cabrerizo, D. Tarragó, C.

Identification of the naturally occurring genes encoding carbapenem-hydrolysing oxacillinases from Acinetobacter haemolyticus, Acinetobacter johnsonii,

Detection of a new subgenotype of hepatitis B virus genotype A in Cameroon but not in neighbouring Nigeria J.M. Hübschen, P.O. Mbah, J.C. Forbi, J.A.

Detection of Bartonella henselae and Bartonella quintana by a simple and rapid procedure using broad-range PCR amplification and direct single-strand.

K. S. Ko, T. Kuwahara, L. Haehwa, Y. -J. Yoon, B. -J. Kim, K. -H

L. Zhang, D. Raoult, P.-E. Fournier

The L2b real-time PCR targeting the pmpH gene of Chlamydia trachomatis used for the diagnosis of lymphogranuloma venereum is not specific to L2b strains

L.S. Christensen, T.V. Madsen, T. Barfod

Isolation of Kingella kingae in the oropharynx during K

Sandfly fever virus outbreak in Cyprus

Three years experience of real-time PCR for the diagnosis of Q fever

Molecular epidemiology of hepatitis B virus in Iran

Comprehensive analysis of mutations in the hepatitis delta virus genome based on full- length sequencing in a nationwide cohort study and evolutionary.

Novel West Nile virus lineage 1a full genome sequences from human cases of infection in north-eastern Italy, 2011 L. Barzon Clinical Microbiology and.

Presentation transcript:

The largest outbreak of hand; foot and mouth disease in Singapore in 2008: The role of enterovirus 71 and coxsackievirus A strains Yan Wu, Andrea Yeo, M.C. Phoon, E.L. Tan, C.L. Poh, S.H. Quak, Vincent T.K. Chow International Journal of Infectious Diseases Volume 14, Issue 12, Pages e1076-e1081 (December 2010) DOI: 10.1016/j.ijid.2010.07.006 Copyright © 2010 International Society for Infectious Diseases Terms and Conditions

Figure 1 Distribution of clinical features in EV71 and non-EV71 patients. International Journal of Infectious Diseases 2010 14, e1076-e1081DOI: (10.1016/j.ijid.2010.07.006) Copyright © 2010 International Society for Infectious Diseases Terms and Conditions

Figure 2 Sequence alignment of 10 outbreak EV71 strains against the hybridization acceptor probe for real-time RT-PCR. The highlighted mismatches may explain the failure of detection of the EV71 strains using this assay. The subgenogroups of the strains, and nucleotide positions at the 5′ (2496) and 3′ (2519) ends of the probe are shown. International Journal of Infectious Diseases 2010 14, e1076-e1081DOI: (10.1016/j.ijid.2010.07.006) Copyright © 2010 International Society for Infectious Diseases Terms and Conditions

Figure 3 Dendrogram constructed based on the complete VP1 gene sequences of 10 outbreak EV71 strains and selected known strains, elucidating B5 and C2 as the respective major and minor EV71 subgenogroups circulating during the 2008 Singapore epidemic. The percentages of replicate trees in which the associated taxa are clustered together are shown next to the branches. Branch lengths are proportional to the number of nucleotide differences. International Journal of Infectious Diseases 2010 14, e1076-e1081DOI: (10.1016/j.ijid.2010.07.006) Copyright © 2010 International Society for Infectious Diseases Terms and Conditions

Figure 4 Alignment of VP1 nucleotides of eight EV71 strains belonging to subgenogroup B5 according to the time of specimen receipt. The disparities at 12 different positions highlight the evolution of the VP1 regions of B5 strains during the course of the large epidemic. For example, five disparities at positions 644, 658, 820, 832, and 866 emerged in strains that were obtained later in the outbreak. Common disparities at nucleotides 19, 373, and 756 were also identified only in strains detected later. International Journal of Infectious Diseases 2010 14, e1076-e1081DOI: (10.1016/j.ijid.2010.07.006) Copyright © 2010 International Society for Infectious Diseases Terms and Conditions

Figure 5 Amino acid sequence variations within the VP1 neutralizing antibody epitopes SP12, SP55, and SP70 of the 2008 outbreak EV71 strains. International Journal of Infectious Diseases 2010 14, e1076-e1081DOI: (10.1016/j.ijid.2010.07.006) Copyright © 2010 International Society for Infectious Diseases Terms and Conditions

Figure 6 Weekly incidence of hand, foot and mouth disease in Singapore during 2008 and 2009. The dotted line depicts the epidemic threshold of 665 cases (www.moh.gov.sg/mohcorp/statisticsweeklybulletins.aspx). International Journal of Infectious Diseases 2010 14, e1076-e1081DOI: (10.1016/j.ijid.2010.07.006) Copyright © 2010 International Society for Infectious Diseases Terms and Conditions