318 bp insertion between Exon 2 and 3

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Presentation transcript:

318 bp insertion between Exon 2 and 3 Identification of five processed SHP2 pseudogenes in the human genome SH2 SH2 PTP Domain Exon 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 SHP2 Gene (PTPN11) 1 4 5 55 56 110 111 175 176 213 214 252 253 284 285 311 312 364 365 407 408 458 459 482 483 532 533 571 572 593 5’ UTR 3’ UTR 1 Thr411 593 SHP2 cDNA 5’UTR ATG TAG 3’UTR -208 1 1179 AAAA 1 224 280 593 SHP2-P3 Pseudogene 94,3% Identity ATG TAG -208 312 bp insertion AAAA 171 283 593 SHP2-P4 Pseudogene 95,0% Identity ATG TAG 513 AAAA 1 54 111 175 593 SHP2-P5 Pseudogene Sequence from Exon 4 deleted 94,8% Identity ATG TAG -203 AAAA SHP2-P6 Pseudogene 5 17 593 Sequence from Exon 1 Deleted 92,6% Identity TAG 15 SHP2-P8 Pseudogene 1 4 56 593 93,9% Identity ATG TAG -201 318 bp insertion between Exon 2 and 3 > 1000 bp insertion http://ptp.cshl.edu or http://science.novonordisk.com/ptp Andersen et al (FASEB J) 2003