National Nutrition Phenotype Database Apologies, welcome, introductions Minutes of the last meeting Matters arising Consortium Agreement Accessibility to the data Website and communications Report from the three clusters Metabolically challenged cohort Elderly cohort National representative sample Agree points of action Date and place of next meeting Any other business

National Nutrition Phenotype Database Apologies, welcome, introductions Minutes of the last meeting Matters arising Consortium Agreement Accessibility to the data Website and communications Report from the three clusters Metabolically challenged cohort Elderly cohort National representative sample Agree points of action Date and place of next meeting Any other business

National Nutrition Phenotype Database Apologies, welcome, introductions Minutes of the last meeting Matters arising Consortium Agreement Accessibility to the data Website and communications Report from the three clusters Metabolically challenged cohort Elderly cohort National representative sample Agree points of action Date and place of next meeting Any other business

Standard FP7 Consortium Agreement

National Nutrition Phenotype Database Apologies, welcome, introductions Minutes of the last meeting Matters arising Consortium Agreement Accessibility to the data Website and communications Report from the three clusters Metabolically challenged cohort Elderly cohort National representative sample Agree points of action Date and place of next meeting Any other business

Data access Limited Open access

National Nutrition Phenotype Database Apologies, welcome, introductions Minutes of the last meeting Matters arising Consortium Agreement Accessibility to the data Website and communications Report from the three clusters Metabolically challenged cohort Elderly cohort National representative sample Agree points of action Date and place of next meeting Any other business

Marine Functional food Dairy Functional food National Diet survey www.foodandhealthresearch.ie National Nutrition Phenotype Database

About nutritional phenotypes The partners Contact us National Nutrition Phenotype Database About nutritional phenotypes The partners Contact us

National Nutrition Phenotype Database Apologies, welcome, introductions Minutes of the last meeting Matters arising Consortium Agreement Accessibility to the data Website and communications Report from the three clusters Elderly cohort Metabolically challenged cohort National representative sample Agree points of action Date and place of next meeting Any other business

NNPD Metabolically-Challenged Cohort Study Ethics proposal submission: 19th May 2008 Estimated start date: September 2008 Estimated duration of study: 36 months

Study Objective To create a component of the national nutritional phenotype database that represents the metabolic stress from varying body-weight overlaid by the additional metabolic stress of a test meal

Glucose Fat Δ 1 4

Develop central hypotheses (examples) - Effects of a metabolic challenge on the metabolomic profile. - How do metabolomics profiles change with time in the post-prandial state? - Is clustering of baseline metabolomic data still evident in the post- prandial data? - Can we identify a metabolic phenotype with respect to post-prandial response using baseline metabolomics?

#1. Options for Study Design 600 x 1 as in the grant or 200 X 2 + 200 x 1 or 100 x 3 + 100 x 2 + 100 x1

#1. Options for Study Design 200 subjects visited once + 200 subjects visited twice = 600 EVENTS 0 1 2 3 4 RECRUITMENT CONTROL DIET CLINICAL INVESTIGATION MORNING (CIM) 200 Norm (Measurements + Test Drink) 200 Overweight 200 Obese Phase 1 Phase 2 0 1 2 3 4 5 RECRUITMENT CONTROL DIET CIM CONTROL DIET CIM 100 Norm 100 Overweight 100 Obese

#2. Inclusion & exclusion Wide inclusion, narrow exclusion preferred

#3 ~ Recruitment Wide: Email/ Companies/ Hospital/ GP practices… - Males/females 18-60 yrs (focus: 25-45yr) - Wide BMI range 18.5 – 45kg.m2 200 Norm 200 Overweight 200 Obese - “Healthy” (NO medication, except for contraceptive pill) …

#4 ~ Test meal Test meal Test Meal 1 only or two Liquid (preferred) Solid Mixed

#5 ~ Measurements: Clinical Investigation Morning Transport Volume Analytes Frequency Storage Collection Processing

#6 Control Diet Objective: To minimise variability between subjects prior to starting. Weight should remain stable during this period Options: Total 4 week run in control Provision of a list of “authorised”/ “non-authorised” Foods and Drinks (Preferred) No control