Have your enzyme lab write up out on your table. If you did not finish it you need to let me know ASAP. Everyone that did finish should move to your table’s computer. Your task is to type up the final lab by going through the answers and creating the best answer for each question. You are NOT just picking one persons answers. You should put the most updated procedure in. This means if you edited the procedure by hand you should include these edits. When you are done. Print it & staple everyone's enzyme write up to the back You have 30 minutes or less. If you finish early work on something for another class.

Factors that effect enzymes… In your lab you tested the effect of different factors on enzymes. To REALLY understand what is happening we will do a simple demonstration with toothpicks.

Toothpickase Demo 1 person in your group will break the toothpicks completely in ½ using only 3 fingers (cannot use the table as leverage) You may not look at the table

Enzyme Substrate Active site Products Transition state Person Toothpickase Demo Enzyme Substrate Active site Products Transition state Person Toothpicks 3 fingertips Broken toothpicks Moment of breaking

Situation #1 – Low Concentration Spread 25 toothpicks around the table. Use the full area of the table “enzyme” person (with eyes closed) has 15s to break as many toothpicks as possible toothpicks If you increase the substrate will you speed up the reaction?

Situation #2 – High Concentration Concentrate 25 toothpicks in front of the person who will play the enzyme. Only break one toothpick at a time! You have 15s to break toothpicks No. If you increase the amount of enzyme it will speed up the reaction

Situation #3 – Cold Temperatures This person should have held their hand in the ice bath for about 4-5 minutes Concentrate 25 toothpicks in front of their seat They have 15s to break toothpicks Slows it down

Situation #4 - Denaturing Excessive heat, acid/base, salt concentration disrupts the bonding of the enzyme, breaking it apart. The protein looses it’s normal shape.

Situation #4 - Denatured Cross your middle and index fingers. Try to break the toothpick (you may not use the table to break it) You have 15s Doesn’t work.

What exactly IS denaturing?

Remember: Enzyme activity depends on shape

Denaturation Thus a change in shape (denaturing) means a non-functional active site and no catalysis.

Protein Shape

Formed by Weak Bonds Held together by weak bonds (ionic bonds, hydrogen bonds, hydrophobic interactions)

How Acid/Base/Salts break those bonds

Denaturation

Enzymes Worksheet Complete from knowledge/memory first in one color Then fill in with notes/questions/help I recommend pencil http://www.youtube.com/watch?v=NdMVRL4oaUo enzyme song Finish for HW