Catalyst What are the subunits of proteins? What organelle makes proteins? What type of bonds hold amino acids together in proteins?

Read + pre-lab questions

Pre-lab questions

PRIMARY SECONDARY v TERTIARY QUATERNARY

protein

How do you think the message gets from the nucleus to the cytoplasm!? Think, Pair, Share Genes that are made of DNA are the instructions for making proteins. DNA is found in the nucleus. BUT! Proteins are made by ribosomes in the cytoplasm. How do you think the message gets from the nucleus to the cytoplasm!?

2 steps in protein synthesis Make an mRNA strand with the DNA message Nucleus

SINGLE DOUBLE RIBOSE A, U, G, C A, T, G, C

U A G G C U C A A

10 min.

2 steps in protein synthesis Ribosome reads the mRNA and makes a protein Cytoplasm - ribosome

Translation animation!

Translation Animation (again!)

Remember: codons = 3 bases Example: AGU= GCC=

Codon Practice 5 min. with partner U4-13 1. Name the amino acids that are coded by the following codons: a. AAA e. CAC b. UAC f. UGA c. GGG g. AGC d. GGA h. CCC 2. Write the amino acid sequence to make up the protein: GCA - GGU - CCA - AUG – UGC 3. Write the amino acid sequence to make up the protein: GCA - GGU - CCG - AUA - UGC

Example: - CCC = __________ - CCU = __________ - CCG = __________ 1 1 neutral See for yourself! Example: - CCC = __________ - CCU = __________ - CCG = __________ - CCA = __________ Original DNA: G G G = ______ Mutated DNA: G G A = ______

What is this stop codon?!

What is this start/initiator codon?! Always AUG

10 min

Shout outs must be written in the first 5 minutes…submit them!! Catalyst Shout outs must be written in the first 5 minutes…submit them!! This is the primary structure of a protein. What do the yellow circles represent? What do the blue lines represent? Draw an example of a folded protein. This molecule is made inside a cell. Which organelle “synthesizes” it?

protein

How do you think the message gets from the nucleus to the cytoplasm!? Think, Pair, Share Genes that are made of DNA are the instructions for making proteins. DNA is found in the nucleus. BUT! Proteins are made by ribosomes in the cytoplasm. How do you think the message gets from the nucleus to the cytoplasm!?

2 steps in protein synthesis Make an mRNA strand with the DNA message Nucleus

SINGLE DOUBLE RIBOSE A, U, G, C A, T, G, C

U A G G C U C A A

10 min.

mRNA codon codon codon

2 steps in protein synthesis Ribosome reads the mRNA and makes a protein Cytoplasm - ribosome

Translation animation!

Translation Animation (again!)

Remember: codons = 3 bases Example: AGU= GCC=

Codon Practice 5 min. with partner U5-5 1. Name the amino acids that are coded by the following codons: a. AAA e. CAC b. UAC f. UGA c. GGG g. AGC d. GGA h. CCC 2. Write the amino acid sequence to make up the protein: GCA - GGU - CCA - AUG – UGC 3. Write the amino acid sequence to make up the protein: GCA - GGU - CCG - AUA - UGC

Example: - CCC = __________ - CCU = __________ - CCG = __________ 1 1 neutral See for yourself! Example: - CCC = __________ - CCU = __________ - CCG = __________ - CCA = __________ Original mRNA: G G G = ______ Mutated mRNA: G G A = ______

What is this stop codon?!

What is this start/initiator codon?! Always AUG

10 min

Exit ticket IN YOUR OWN WORDS explain how protein synthesis occurs in a cell. Underline the following words in your response: Nucleus Polypeptide/protein Ribosome Transcription DNA/gene Translation Amino acid Codon mRNA Anticodon tRNA

This is the gene (DNA) that makes hemoglobin, a protein in your blood. Catalyst This is the gene (DNA) that makes hemoglobin, a protein in your blood. A T G T A C C G A A A T Transcribe the gene into mRNA: Translate the mRNA into protein:

U5-8

U5-9

Insertion deletion substitution DNA sequence negative Insertion deletion substitution Pg U5-10

Tay-Sach’s Disease

deleted inserted AAA CCT TTG GG AAA CCC ATT TGG G ACA CCC TTT GGG

CFU What is a mutation? What are the 3 types of mutations? CGA TTC GGA TGA The above DNA strand undergoes a DELETION mutation at the END. Write the mutated strand.

Sickle cell anemia

Protein Synthesis Practice…again! Pg U5-12 & U5-13 Staple these into your unit 5 packets!

MQ1 5 minutes to review your notes & practice! Protein Synthesis MQ1 5 minutes to review your notes & practice!

Picture word bank mRNA Transcription Translation tRNA Amino acid Protein/polypeptide Ribosome

catalyst If you could clone (make a copy) of any LIVING thing – what would you clone? Why? If you could pick your baby’s characteristics (physical traits) would you?

ON OFF environment expressed needs genes environment U5-11.5 ON OFF environment expressed needs genes environment traits

Biotechnology 5 “stations” 4 minutes each Fill in your notes!

During the videos, fill out the corresponding reflection questions for each type of biotechnology

Biotechnology

Human Genome Project

Gene Therapy

Stem Cell Research

Cloning

Work Time Complete ALL unfinished practice in your packet. U5-6 through U5-13 should be completed by the end of today. Work together. DO NOT copy. Copying = plagiarism = 0

catalyst a. b. c. Where does transcription happen in the cell? Where does translation happen in the cell? What are a, b, and c in the labeled picture? a. b. c.

catalyst a. b. c. Where does transcription happen in the cell? Where does translation happen in the cell? What are a, b, and c in the labeled picture? a. b. c.

catalyst Turn in your U5 Vocab! (or a pink slip!) What is molecule 1? What process is occurring at arrow #1?   What process is occurring at arrow #2? #1 #2

genetic DNA DNA one insulin bacterial

fragments size compare relationships

10 minutes

Biotechnology Review your notes for 5 minutes… U5 MQ 2 Biotechnology Review your notes for 5 minutes…

Catalyst Copy the following onto your catalyst sheet and then match: Column 1 Column 2 Transcription Replication Translation DNA  DNA DNA  mRNA mRNA  protein

Unit 5 Review Choose 3: 15 minutes @ each station Fact first: complete the review of protein synthesis U5 Crossword: complete the vocabulary review! Protein Synthesis Online Lab: *computer* Gel Electrophoresis: solve the cases! Know your pictures!: complete the labeling/identification of structures Mr. Lascano: Complete study guide, quiz corrections, and organize your notebooks 15 minutes @ each station

#tbt If a bear’s brain cell has 20 chromosomes, how many chromosomes are in his sperm? Which image below shows mitosis? Which image below shows meiosis?

Unit 5 Test DO NOT WRITE ON THE TEST When you finish: CHECK your answers Turn it in at the front Remain seated and silent