Lab 2 – The Microscope Life Science Lab BSC 1005L.

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Presentation transcript:

Lab 2 – The Microscope Life Science Lab BSC 1005L

The Dissecting Microscope

The Objective Lenses Scanning Objective (4x) Low-power objective (10x) Red color band, smallest one Low-power objective (10x) Yellow color band High-power objective (40x) Blue color band Oil Immersion objective (100x) Not used in this lab

Magnification Magnification = ocular x objective Ocular lense is usually 10 Examples: Under 4x objective – magnification of specimen is 40 x Under 10x objective – magnification of specimen is 100x Under 40x objective – magnification of specimen is 400x

Microscopy Using the Microscope When done: Find the microscope # assigned to you in the cabinet Carry the microscope with BOTH hands back to the lab bench Plug it in, turn on light Place slide on stage Always view specimen on lowest power first! Focus and center Switch to higher power Focus and center, etc. When done: Switch lens to lowest power!! Take slide off Clean lens with only lens paper Turn light off Unplug wrap cord around base Place back in cabinet

Things to Remember Be careful with Microscope Never turn head around! Always clean lenses with lens paper In your drawer You can clean slides with Kimwipes Always put slides back in slide box in numerical order!!!!!

Learn parts of the compound microscope that we will use Learn how to make a wet mount Refer to Figure 2.9 Metric System Be comfortable with measurements and know how to do conversions!