Good Afternoon! FIND YOUR NAME ON THE POST-IT NOTES Warm Up- Today: Grab the two sheets on the front desk. Begin answering the questions using the lab cartoon. Pass forward your homework. (book WS) Today: Microscope notes Finish lab View microscope slides Class work

Good Afternoon Warm Up: Today: Microscope labeling on front desk…Do not use your notes! Today: Characteristics of Life notes Class work Microscope Quiz

The Compound Light Microscope

What is a compound light microscope? Mechanism for enlarging an image

Magnification The two lenses are called: Eyepiece Objective The eyepiece has a magnification of “10x” The magnification of the objective lenses vary and are marked on the lens Total magnification = (Eyepiece) X (Objective) Example: 10 X 40 = 400

What is the Total Magnification? What’s my power? What is the Total Magnification? 400x

The Parts of the Microscope and Their Function Maintains proper distance between lenses Magnification Rotates the objectives Support body tube Magnification Low, medium,high(greatest magnification) Supports slide Holds slide In place Moves stage up- down/Focuses image Regulates amount of light Sharpens the image Reflects light towards eyepiece Supports microscope

Proper Procedures Carry the microscope with one hand under the base while grasping the arm with the other hand Be careful of the cord when moving around the lab tables!!

Proper Procedures 2. Rotate the nosepiece to obtain the proper objective lens Make sure the lens clicks into place!! 3. Look through the eyepiece and switch on the light This is your field of view!

Do not use course adjustment with high power! Proper Procedures 4. Place slide on stage, center specimen over stage 5. Locate specimen on scanning or low power using coarse adjustment 6. Carefully switch to high power 7. Focus image with fine adjustment ONLY on high power!!!!!!! Avoid damaging slide! Do not use course adjustment with high power!

Storing the Microscope Four steps prepare the microscope for storage: the 10X objective is in place the stage is all the way down the power is off the cord is wrapped around the base

You do not need to use the stage clips when viewing wet-mount slides! How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. 4 - Lower the cover slip on top of the drop to prevent air bubbles Cover Slip Lower slowly You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the lowest power objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

Homework QUIZ Friday! Characteristics packet due Tuesday Microscope notes Characteristics packet due Tuesday

GOOD MORNING!!! I’ll collect your homework. No Warm Up today Today: Notes Class work QUIZ TOMORROW

Let’s Review! Eyepiece Body tube Nosepiece Arm Low Medium Stage 9. 10. 11. 14. Eyepiece 1. 2. 3. 4. 5. 6. 7. 8. Body tube Nosepiece Arm Low Medium Stage High power Stage clips 12. Course adjustment Diaphragm 13. Fine adjustment Light source Base

So Let’s Review Let’s Review! Eyepiece Body tube Nosepiece Arm Scanning Low Power Stage High power Stage clips Course adjustment Diaphragm Fine adjustment Light source Base

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

References http://www.cerebromente.org.br/n17/history/neurons1_i.htm Google Images http://science.howstuffworks.com/light-microscope1.htm

Evolution of the microscope Choice of lens magnification 2 set lenses

Anthony van Leeuwenhoek The History Hans and Zacharias Janssen of Holland in the 1590’s created the “first” compound microscope Anthony van Leeuwenhoek and Robert Hooke made improvements by working on the lenses Robert Hooke 1635-1703 Anthony van Leeuwenhoek 1632-1723 Hooke Microscope

The History Zacharias Jansen 1588-1631 The “First” Microscope