R.L. Proietto, L.S. Hinckley, L.K. Fox, S.M. Andrew

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Evaluation of a clay-based acidic bedding conditioner for dairy cattle bedding R.L. Proietto, L.S. Hinckley, L.K. Fox, S.M. Andrew Journal of Dairy Science Volume 96, Issue 2, Pages 1044-1053 (February 2013) DOI: 10.3168/jds.2012-6020 Copyright © 2013 American Dairy Science Association Terms and Conditions

Figure 1 Least squares means and standard errors for (a) total gram-negative bacteria, (b) environmental streptococci, and (c) coliform bacteria isolated from untreated sawdust (CON; white bars) or sawdust treated with a clay-based acidic bedding conditioner (ABC; black bars). Measurements were taken at d 0, 1, 2, 7, 14, and 21 over 2 periods. *P<0.05. Journal of Dairy Science 2013 96, 1044-1053DOI: (10.3168/jds.2012-6020) Copyright © 2013 American Dairy Science Association Terms and Conditions

Figure 2 Least squares means and standard errors for the pH of untreated sawdust (CON; white bars) or sawdust treated with a clay-based acidic bedding conditioner (ABC; black bars). Measurements were taken at d 0, 1, 2, 7, 14, and 21 during (a) period 1 and (b) period 2. *P<0.05. Journal of Dairy Science 2013 96, 1044-1053DOI: (10.3168/jds.2012-6020) Copyright © 2013 American Dairy Science Association Terms and Conditions

Figure 3 Least squares means and standard errors for the bedding cleanliness of untreated sawdust (CON; white bars) or sawdust treated with a clay-based acidic bedding conditioner (ABC; black bars). Measurements were taken 3 times daily during (a) period 1 and (b) period 2. Cleanliness scores were on a 0 to 5 scale, where 0 = clean, 5 = extremely soiled. Journal of Dairy Science 2013 96, 1044-1053DOI: (10.3168/jds.2012-6020) Copyright © 2013 American Dairy Science Association Terms and Conditions

Figure 4 Least squares means and standard errors for streptococci isolated from teat-end swabs of 16 lactating Holstein cows bedded on untreated sawdust (CON; white bars) or sawdust treated with a clay-based acidic bedding conditioner (ABC; black bars). Measurements were taken at d 0, 1, 2, 7, 14, and 21 during (a) period 1 and (b) period 2. *P<0.05. Journal of Dairy Science 2013 96, 1044-1053DOI: (10.3168/jds.2012-6020) Copyright © 2013 American Dairy Science Association Terms and Conditions

Figure 5 Least squares means and standard errors for coliform bacteria isolated from teat-end swabs of 16 lactating Holstein cows bedded on untreated sawdust (CON; white bars) or sawdust treated with a clay-based acidic bedding conditioner (ABC; black bars). Measurements were taken at d 0, 1, 2, 7, 14, and 21 during (a) period 1 and (b) period 2. *P<0.05. Journal of Dairy Science 2013 96, 1044-1053DOI: (10.3168/jds.2012-6020) Copyright © 2013 American Dairy Science Association Terms and Conditions

Figure 6 Least squares means and standard errors for Klebsiella spp. isolated from teat-end swabs of 16 lactating Holstein cows bedded on untreated sawdust (CON; white bars) or sawdust treated with a clay-based acidic bedding conditioner (ABC; black bars). Measurements were taken at d 0, 1, 2, 7, 14, and 21 during (a) period 1 and (b) period 2. *P<0.05. Journal of Dairy Science 2013 96, 1044-1053DOI: (10.3168/jds.2012-6020) Copyright © 2013 American Dairy Science Association Terms and Conditions