Planting Science- Round up Taylor Driscoll Sam Carolin Vanessa Lomerson Sarah Hartman

Work cited * Bulman, Christopher A., et al. "Perturbations of amino acid metabolism associated with glyphosate-dependent inhibition of shikimic acid metabolism affect cellular redox homeostasis and alter the abundance of proteins involved in photosynthesis and photorespiration." Plant Physiology 157.1 (2011): 256+. Science in Context. Web. 25 Mar. 2015 * Host, Laurie A. "Detecting Genetically Modified Food by PCR." The American Biology Teacher 74.2 (2012): 128. Science in Context. Web. 30 Mar. 2015 * "Herbicide-Tolerant Plants." Biotechnology: Changing Life Through Science. Detroit: UXL, 2012. Science in Context. Web. 30 Mar. 2015.

Background We all have used a herbicide on our plants in order to kill the weeds around it. The herbicide Roundup has been used on plants for years now. Going from many plants like corn to beans. Many scientist have looked at what type of effects the herbicide roundup has on plants. There is two types of ways to use Roundup. A genetically modified bean, which has the roundup herbicide in its genes in order to help it grow while killing weeds without killing itself. Also spraying the herbicide by hand. The Roundup herbicide has effected beans by resisting pesticides and weeds to attack the plant, has increased nutritional value, and stress tolerance. The plants that are genetically engineered with herbicide- tolerance are able to survive. However, there have been cases of non genetically modified plants that were sprayed with herbicide and began to die after being sprayed. If the plant is sprayed past the flowering stage it causes problems and effects the growing of the plant. To get good results it is said to spray 64oz for 25 gallons and spray twice, to ensure the plants grow with no weeds, but also keep the herbicide from killing the plant itself.

Purpose/Hypothesis Purpose: At what concentration does the herbicide round up ready, affect the rate of photosynthesis on bean plants? Hypothesis: If the plants are treated with round up ready, then they will not produce as much oxygen as the control plant, and the rate of photosynthesis will decrease.

Procedure Experiment Plant four plants for each concentration Label the cups 0, 5, 15, 25 (Percentage on cup represents the percentage of herbicide in water) Water plants 30 ml until they begin to grow After the plants begin to grow start adding the proper amount of concentration Roundup into water Keep watering the plants everyday 30 ml with concentration of Roundup that matches the percentage labeled on the cup

Procedure Leaf Disk Assay 1.Cut 10 disks from each plant 2.Create solution with water and baking soda 3. Put disks in syringe with solution and create a vacuum to make disks sink 4. Place disks back in solution. 5. Place in a light box. 6. Record length of time each disk takes to rise.

Graphs 4 minutes 46 minutes 50 minutes Never

Graphs 5% concentration t=0.723 15% concentration t= 0.983

Limitations and Implications During the FLDA the sun was not out More Trials Leaf disks began to stick together Bugs eating one plant Implications The results can only determine the effects of herbicide on a bean plant. To further the experiment it would be helpful to test other types of plants and record data. The herbicide could also be added in different ways such as adding it into the soil instead of the water.