2009. 04. 14 NJSDGP003_부미경 PCR 1. Sample list 2. Primer list.

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2009. 04. 14 NJSDGP003_부미경 PCR 1. Sample list 2. Primer list

NJSDGP003_부미경 PCR 3. PCR condition 3-1. PCR Condition 2009. 04. 14 NJSDGP003_부미경 PCR 3. PCR condition 3-1. PCR Condition 3-3. PCR Reaction Combination Temp. Time Cycles Final Reaction vol. 94 ℃ 5 min 1 25ul 30 sec 30 55 ℃ 1 min 72 ℃ 72℃ 7 min 4℃ Storage

NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-arc 2% 0.5x TAE gel 2009. 04. 14 NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-arc 1 2 3 4 5 6 7 8 9 10 11 12 13 1415 16 17 18 1920 21 22 23 24 25 2627 28 29 30 31 32 33 34 35 36 37 38 39 4041 42 43 4445 46 47 48 4950 51 52 5354 55 56 5758 59 60 6162 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-aroE 2% 0.5x TAE gel 2009. 04. 14 NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-aroE 1 2 3 4 5 6 7 8 9 10 11 12 13 1415 16 17 18 1920 21 22 23 24 25 2627 28 29 30 31 32 33 34 35 36 37 38 39 4041 42 43 4445 46 47 48 4950 51 52 5354 55 56 57 58 59 60 61 62 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-glpF 2% 0.5x TAE gel 2009. 04. 14 NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-glpF 1 2 3 4 5 6 7 8 9 10 11 12 13 1415 16 17 18 1920 21 22 23 24 25 2627 28 29 30 31 32 33 34 35 36 37 38 39 4041 42 43 4445 46 47 48 4950 51 52 5354 55 56 5758 59 60 6162 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-gmk 2% 0.5x TAE gel 2009. 04. 14 NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-gmk 1 2 3 4 5 6 7 8 9 10 11 12 13 1415 16 17 18 1920 21 22 23 24 25 2627 28 29 30 31 32 33 34 35 36 37 38 39 4041 42 43 4445 46 47 48 4950 51 52 5354 55 56 5758 59 60 6162 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-pta 2% 0.5x TAE gel 2009. 04. 14 NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-pta 1 2 3 4 5 6 7 8 9 10 11 12 13 1415 16 17 18 1920 21 22 23 24 25 2627 28 29 30 31 32 33 34 35 36 37 38 39 4041 42 43 4445 46 47 48 4950 51 52 5354 55 56 5758 59 60 6162 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-tpi 2% 0.5x TAE gel 2009. 04. 14 NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-tpi 1 2 3 4 5 6 7 8 9 10 11 12 13 1415 16 17 18 1920 21 22 23 24 25 2627 28 29 30 31 32 33 34 35 36 37 38 39 4041 42 43 4445 46 47 48 4950 51 52 5354 55 56 5758 59 60 6162 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-yqiL 2% 0.5x TAE gel 2009. 04. 14 NJSDGP003_부미경 PCR 4. The Result of PCR- Primer-yqiL 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 1819 20 21 22 23 24 25 2627 28 29 30 31 32 33 34 35 36 37 38 39 4041 42 43 4445 46 47 48 4950 51 525354 55 56 5758 59 60 6162 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-arc purify 후 2009. 04. 14 NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-arc purify 후 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 50ng 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 50ng 57 58 59 60 61 62 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-aroE purify 후 2009. 04. 14 NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-aroE purify 후 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 50ng 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 50ng 57 58 59 60 61 62 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-glpF purify 후 2009. 04. 14 NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-glpF purify 후 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 50ng 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 50ng 57 58 59 60 61 62 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-gmk purify 후 2009. 04. 14 NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-gmk purify 후 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 50ng 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 50ng 57 58 59 60 61 62 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-pta purify 후 2009. 04. 14 NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-pta purify 후 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 50ng 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 50ng 57 58 59 60 61 62 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-tpi purify 후 2009. 04. 14 NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-tpi purify 후 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 50ng 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 50ng 57 58 59 60 61 62 63 2% 0.5x TAE gel

NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-yqiL purify 후 2009. 04. 14 NJSDGP003_부미경 PCR 5. The Result of PCR- Primer-yqiL purify 후 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 50ng 41 42 43 44 45 46 47 48 57 58 59 60 61 62 63 49 50 51 52 53 54 55 56 50ng 2% 0.5x TAE gel