Max R. Bangs*; Marlis R. Douglas^; Michael E. Douglas^

Slides:

Advertisements

Similar presentations

Idaho Department of Fish and Game Clearwater Region Lewiston Idaho EVALUATE AND CONTROL BROOK TROUT POPULATIONS IN NORTHCENTRAL IDAHO WATERSHEDS : ADDRESSING.

Advertisements

The bead maze: Adapting the water maze task to the human manipulatory scale E.L. Loda, A.B. Thoennes, J.R. Köppen, S.S. Winter, D.A. Hamilton, D.G. Wallace.

Admixture in Horse Breeds Illustrated from Single Nucleotide Polymorphism Data César Torres, Yaniv Brandvain University of Minnesota, Department of Plant.

Restoring Life History Diversity to Bonneville Cutthroat Trout and Bluehead Sucker in the Weber River Paul Burnett – Trout Unlimited Ben Nadolski – Utah.

Step 1: Valley Segment Classification Our first step will be to assign environmental parameters to stream valley segments using a series of GIS tools developed.

Background The demographic events experienced by populations influence their genealogical history and therefore the pattern of neutral polymorphism observable.

Phylogeographic relationships of Cottus bairdii Sun Yeong Oh 1, Peter Unmack, Dennis K. Shiozawa 1, R. Paul Evans 2, Nina Laitinen 1 1 Department of Biology,

Characterization of Endophytic Cellulose Degrading Fungi at the Sevilleta LTER site Zachary T. Gossage 1, Carolyn Weber 2, Cheryl Kuske 2, and Andrea Porras-Alfaro.

IPM Issues in the West Kevin Fitzsimmons University of Arizona Department of Soil, Water and Environmental Science.

Determining the scale of biologically important local adaptation in Atlantic salmon using a common garden experimental approach Ciar O’Toole 2 nd Year.

The Field Office Technical Guide and Other Technical Resources CNMP Core Curriculum Section 2 — Conservation Planning.

Microbial Community Biomarker in Barnegat Bay Evangelina Pena 1, Lora McGuinness 1, Gary Taghon 1, Lee Kerkhof 1 Introduction Efforts to remediate anthropogenic.

Watershed assessment, management and restoration of Little Kern golden trout in the Little Kern River, California Acknowledgements Assess the current status.

Background  The soft shell clam, Mya arenaria, currently occupies a large geographical range in the northern hemisphere.  Soft shell clams are found.

Howard Hughes Medical Institute-NMSU Research Scholar

Banbury october 2007 Michel Veuille Ecole Pratique des Hautes Etudes - Paris1 Can we extend intraspecific population genetics to community population.

Predation and the use of Tamarisk as a nesting substrate by Southwestern Willow Flycatchers (Empidonax traillii extimus) Stephanie Muise, Katie Stumpf.

A Study of Painted Turtles (Chrysemys picta) in Lake Marburg at Codorus State Park Olivia Yaple, Department of Biology, York College Introduction Methods.

Development and validation of models to assess the threat to freshwater fishes from environmental change and invasive species PIs: Craig Paukert Joanna.

“Recent next generation sequencing results” MACHADO LAB.

Effect of Hydrogen Peroxide Treatment on Ability to Perform Alu Polymerase Chain Reaction in Hair Samples By: Dominic Flaim Department of Biological Sciences,

Conservation Genetics of the Plains Topminnow, Fundulus sciadicus The plains topminnow (Fundulus sciadicus) is a freshwater killifish endemic to the Great.

PHYLOGEOGRAPHY OF THE BOBWHITES Damon Williford, Randy W. DeYoung, Leonard A. Brennan, Fidel Hernández Caesar Kleberg Wildlife Research Institute Texas.

Morphospace plot of Insectivore Mammals’ Mandibles; Preliminary evidence for morphological convergence and disparity Method We photographed the skulls.

MALE REPRODUCTIVE SUCCESS IN SOUTHERN ELEPHANT SEALS BEHAVIOURAL ESTIMATES AND GENETIC PATERNITY INTRODUCTION The southern elephant is the species with.

Experimental Design and Data Structure Supplement to Lecture 8 Fall

Susannah Woodruff, Rob Lonsinger, Lisette Waits Fish and Wildlife Sciences, University of Idaho MONITORING SPECIES OF CONCERN ON MILITARY LANDS USING NONINVASIVE.

Type Your Poster Title in Here School name and Watershed name (ie. Sand Hill Watershed) Date Insert school logo up here Picture of team? Elevation Profile.

Results I) Regional Survey Rarefaction curves leveled off across sites, suggesting that the sample effort was sufficient to capture differences between.

Characterization of a Local Population of Black-capped Chickadees (P. carolinensis), Carolina Chickadees (P. atricapillus), and their hybrids – a preliminary.

Tardigrade Identification and Distribution at York College using Molecular Techniques Jason Lamontagne, York College of PA, Dept. of Biological Sciences.

Radial growth in Pinus contorta relative to changing climate patterns in British Columbia: Genetic response to annual climate variations, Sierra.

Female dispersal and gene flow Low High Male dispersal and gene flow Low High Geographic structure in… mtDNA-- yes Autosomal genes-- yes Y-linked genes--

Fecal DNA typing to determine the fine scale population structure and sex-biased dispersal pattern of Eurasian otter (Lutra lutra) in Kinmen CHUAN-CHIN.

Bat Occurrence and Habitat Selection on the Delmarva Peninsula Andrew McGowan.

Introduction Methods Results and Discussion Collin Ahrens and Carol Auer Department of Plant Science University of Connecticut Drought and Salinity Tolerance.

The Bovine Genome Sequence: potential resources and practical uses. Nicola Hastings, Andy Law and John L. Williams * * Department of Genetics and Genomics,

Factsheet # 26 Understanding multiscale dynamics of landscape change through the application of remote sensing & GIS CREATING LIDAR-DRIVEN MODELS TO IMPROVE.

Simple-Sequence Length Polymorphisms

Department of of Plant Science CONTEXT AND QUESTIONS OF THE RESEARCH

Number of Sample Sequenced and analyzed

ASSESSMENT OF GENETIC VARIATION IN CACAO CLONES COLLECTION OF

Abstract Tables & Figures Introduction Materials & Methods Results

SUNY Korea BioData Mining Lab - Journal Review

Introduction Conclusion References Aim of the work

Cryptic Sucker Species of the Northeast

Kendra David and Dr. Hayley Lanier

N. W. Byer1,3, B. N. Reid1,4, R. A. Seigel2, and M. Z. Peery1

Max Bangs, Marlis Douglas, & Michael Douglas

Sierra M. Love Stowell & Andrew P. Martin Student Figures

Teja Petra Muha¹, Carlos Garcia De Leaniz¹, Sonia Consuegra del Olmo¹

RESULTS and DISCUSSION

Observed Heterozygosity Expected Heterozygosity

Charlotte Levy1 & Eloise Brown2

Statistical Methods for Model Evaluation – Moving Beyond the Comparison of Matched Observations and Output for Model Grid Cells Kristen M. Foley1, Jenise.

Decline of the Savannah River Redeye Bass (Micropterus coosae) due to Introgressive Hybridization with Invasive Alabama Spotted Bass (Micropterus punctulatus.

Max Bangs, Joe Quattro, Ken Oswald, and Jean Leitner

TABLE 1. ENVIRONMENTAL VARIABLE SUITE

Relationship between Genotype and Phenotype

The Ashkenazic Jewish Bloom Syndrome Mutation blmAsh Is Present in Non-Jewish Americans of Spanish Ancestry Nathan A. Ellis, Susan Ciocci, Maria Proytcheva,

COLLECTION SITE MYEIK, MYANMAR

Alycia L. Stigall, Department of Geological Sciences, Ohio University

Sequencing of t(2;7) Translocations Reveals a Consistent Breakpoint Linking CDK6 to the IGK Locus in Indolent B-Cell Neoplasia Edward P.K. Parker, Reiner.

Track the Split of Crocodile Sub Populations

Evolutionary History of the ADRB2 Gene in Humans

Lecture 19 – Non-tree Based Methods

Relationship between Genotype and Phenotype

Representative results showing PCR amplification of DNA from stools using PCR primers amplifying (A) SFB sequences and expected to generate an SFB 139 bp.

Presentation transcript:

Max R. Bangs*; Marlis R. Douglas**^; Michael E. Douglas**^ Utilization of Molecular Markers to Examine Potential Hybridization in Bluehead Suckers (Catostomus discobolus) in the Weber River, UT Max R. Bangs*; Marlis R. Douglas**^; Michael E. Douglas**^ *Graduate Student of the Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701 **Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701 ^Illinois Natural History Survey, University of Illinois, Urbana/Champaign, IL 61820 Introduction Fishes of the desert Southwest face numerous anthropogenic threats including introduction of invasive species, habitat modifications, and recent climate change driven drought. Continuous monitoring of threatened taxa provides important data to develop management plans. However, accurate assessments can be inhibited if species identifications are ambiguous due to hybridization. Level of admixture (e.g., hybridization or introgression) is generally difficult to quantify without employing molecular genetic techniques. Douglas et al. screened over 1,500 samples of Catostomus spp. throughout the Southwest using 18 microsatellite loci and two mitochondrial genes. Several of these samples were identified as having admixed ancestry, however, whether these hybrids are introgressing or potentially sterile could not be accurately accessed. We designed a database using three biallelic nuclear loci along with two mitochondrial genes in order to determine if samples identified as putative hybrids were first generation hybrids (a result of two pure parents of different species mating) or introgressed hybrids (a result of a hybrid backcrossing with a parental species). Here we present a study of how this database can be used on the Bluehead Sucker (Catostomus discobolus) in the Weber River, UT (Bonneville Basin), where the species is of conservation concern and hybridization with Utah Sucker (C. ardens) has been recognized as an issue. Methods Samples identified as putative hybrids (n=13) in the Weber River, UT, were screened to determine if introgression is occurring. PCR Amplification: - mtDNA ATPase 6 and 8 genes (842 bps) - three nDNA loci (415, 211, and 323 bps) Direct Sequencing of PCR Products Sequences edited (Sequencher v4.1), aligned (BioEdit), and compared to representative samples of several other sucker species that could be the source of introgressed material. Results We successfully obtained ~1 kb of nDNA sequence across three loci. Of this, there were 66 polymorphic sites with 46 of these sites being diagnostic between species. (Tables 2, 3 ,and 4) All individuals were heterospecific across all three loci, indicating first generation crosses. Coupled with the mtDNA sequence, we were able to characterize the parental species involved as being Utah Sucker female x Bluehead Sucker male 64% of the time and Bluehead Sucker female x Utah Sucker male 36% of the time. uitfwd.com Conclusion Bluehead Sucker and Utah Sucker are hybridizing in the Weber River, UT. No introgression into either parental species was found, suggesting hybrid sterility. No parental bias, thus both types of female/male crosses can produce hybrids. Due to lack of introgression into parental species, management suggestions are for the identification and removal of hybrids. Table 1: Haplotypes for Utah Sucker (UTS) and Bluehead Sucker (BHS) of the three nuclear loci used. Polymorphic sites are to be read vertically. A (.) represents a match to the top haplotype. Fixed differences between species are shown in red. 0 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 3 3 3 6 9 0 3 4 5 5 6 6 6 6 6 6 6 6 6 6 7 8 2 3 0 1 9 5 2 3 9 0 8 9 0 1 2 3 4 5 6 7 8 9 9 4 5 0 1 2 2 UTS 1 G A A T G G T T G G T T A G C T G C T G T A A T UTS 2 . . C . . . . . . . . . . . . . . . . . . . . . BHS 1 A C . C A - - - - - - - - - - - - . A . G T C C BHS 2 A C . C A - - - - - - - - - - - - A A . G T C C BHS 3 A C . C A - - - - - - - - - - - - . . A G T C C 0 0 0 0 0 0 1 1 1 1 1 2 6 9 9 9 9 0 1 3 4 6 5 6 1 3 4 5 0 6 1 2 5 UTS 1 A A G G T G G T C G T UTS 2 . . . . . . . A . . . UTS 3 . . . . . . . . . A . UTS 4 G . . . . . . . . . . BHS 1 . G A T G A . . . . A BHS 2 . G A T G A T . . . A BHS 3 . G A T G A . . A . A 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 4 5 6 7 8 8 9 9 9 0 0 0 0 0 0 1 1 1 1 2 4 4 4 5 5 5 5 5 5 6 9 9 1 1 6 0 4 7 8 9 0 1 2 3 5 6 4 5 6 8 1 7 8 9 0 1 2 3 4 9 1 7 UTS 1 G T T G T C C A G T G C C A C T C T G G T T T G - - - - T - G UTS 2 . . . . . . . . . . . . . . . . . . T . - - - - . . . . . . . UTS 3 . . . . . . . . . . . . . G . . . . T . - - - - . . . . . . . UTS 4 . . . . . . . . . . . . . . . . . . . . . . . . T T T G . . . UTS 5 . . . . . . . . . . . . . . . . . . . . - - - - . . . . . . . BHS 1 T A A A C T - - - - - - - . T - - - . T . . . . . . . . G C A BHS 2 T A A A C T - - - - - - - . T - - - . T . . . . . . . . . C A Future Goals Determine the range and severity of hybridization between these two species in the Bonneville Basin. Determine if there is a correlation between environmental factors (e.g. anthropogenic causes) and occurrence of hybridization. Examine other hybrid events within Catostomus throughout the Southwest. Figure 1: Putative hybrid of a Bluehead Sucker (C. discobolus) from the Weber River, UT. (Picture curtsey of Paul Thompson) Goals Develop a database for screening Catostomus spp. for introgressive hybridization Determine if Bluehead Sucker x Utah Sucker hybrids are introgressing Characterize the parental species involved in admixture Acknowledgements Numerous agencies contributed field expertise, specimens, technical assistance, collecting permits, funding or comments for the completion of this study. First and foremost, we like thank Paul Thompson, Aaron Webber, Cassie Melon and Krissy Wilson from Utah Department of Natural Resources, who initiated this study. Thanks also goes to their team at UDNR, in particular P. Martin, S. Jones, K. Beidinger, M. Breen, T. Hedrick., S. McKay, and C. Walker. Thanks also go to natural resource agencies in Arizona, Colorado, New Mexico, Utah and Wyoming as well as the National Park Service and U.S. Bureau of Reclamation. We are also in debt to the students, postdoctorals and faculty who contributed to the development of our research: A. Albores, W. Anthonysamy, P. Brunner, T. Dowling, R. Cooper, J. Cotter, M. Davis, E. Fetherman, M. Hopken, M. Kwiatkowski, S. Mussmann, A. Reynolds, J. Reynolds, C. Secor, and P. Unmack. Sampling procedures were approved under IACUC permit 98-456R (Arizona State University) and 01-036A-01 (Colorado State University). Funding was provided by a grant from UDNR. Figure 2: Drainage map of Utah. Only major drainages are shown. Circle shows location of Weber River.