Ch.12 Immunology Applications Microbiology
12.1 Immunization Passive Immunization Vaccination Artificial-injection of antibodies Natural-mother to baby Vaccination Introduction of weakened or dead antigens Development of New Vaccines WHO Cancer Vaccine Safety Very low risk No connection to Autism
12.2 Diagnostic Immunology Immunoassays for Disease Lab techniques used to detect antibody production Serology Study of blood serum and other body fluids to identify antibodies Agglutination Reactions Assess the presence of antibodies Neutralization reactions Evaluate neutralizing antibodies
12.2 Diagnostic Immunology Complement Fixation Test for specific antibody Fluorescent Antibodies Tagging, easier identification Enzyme-Linked Immunosorbent Assay (ELISA) Spectrophotometer Immunoblot Chemicals in paper react to certain antibodies
12.2 Diagnostic Immunology In Vivo testing Using animal models of disease to help discover new ways of solving complex health problems Mice and rats most common Future of Diagnostic Immunology Technology-automation Miniaturization Amplified immunoassays Flow cytometry Biotechnology
12.3 Preparations for Diagnosing Infection Specimen collection Blood, urine, cerebrospinal fluid, mucosal swabs, biopsy & necropsy Immediate direct examination Morphology Cultivation DNA analysis Polymerase Chain Reaction (PCR) Nucleic acid sequencing (rRNA)
12.4 Immunity Disorders: Hypersensitivity Type I (Anaphylactic) Reactions Mast cells, hives, shock, epi pen Diagnosis & treatment of allergy Skin prick test Type II (Cytotoxic) reactions Attack own cells Type III (Immune complex) Clogging of vessels Type IV (Delayed Cell-mediated 2-3 days to develop
12.5 Autoimmune Diseases Genetics-75% women Cytotoxic-does not recognize “self” Lupus, T1 diabetes, RA Immune Complex Cell-mediated
12.6 Immunodeficiencies Primary Part of immune system missing Secondary Acquired, AIDS Immunotherapy for cancer Use of the body’s own immune system to reject cancer