FIGURE 23. 1 Mouse Embryonic Stem Cells FIGURE 23.1 Mouse Embryonic Stem Cells. (a) Growth of mES colonies on feeder layers with serum and LIF. (b) Subcultured mES cells in serum and LIF. (c) Isolated inner cell mass (ICM); ‘‘rind and core’’ structure of hypoblast growing round epiblast. (d) Outgrowths of mES cell-like from cultured epiblast (see also Plate 25a). (e, f ) Growth of mES colonies in 2i medium, (e) 10× and (f ) 20× magnification (see also Plate 25b).

FIGURE 23. 2 Human Embryonic Stem Cells FIGURE 23.2 Human Embryonic Stem Cells. (a) Colony of hES cells on feeder layer. Undifferentiated cells around the edge of the colony surround more differentiated cells with a brownish appearance in the center. The colony is surrounded by growth-inactivated mouse embryo feeder cells (see also Plate 25c). (b) Free-floating embryoid bodies (EBs), typically 300 to 500 cells per cluster, generated by detaching colonies from feeder layer and preventing further reattachment (see also Plate 25d). (From Cooke & Minger, 2007).

FIGURE 23. 3 Pulled Glass Pipettes FIGURE 23.3 Pulled Glass Pipettes. Various different pulled tips should be explored to find a preference, for example a straight-edged or rounded-edged pipette tip. (From Cooke & Minger, 2007).

FIGURE 23. 4 Bone Marrow-Derived MSCs FIGURE 23.4 Bone Marrow-Derived MSCs. (a, b) Colonies formed after plating of whole bonemarrow mononuclear cells. Morphology and optimal passaging density of MSCs. (c) An early passage culture of MSCs. (d) A monolayer at the appropriate density for passage. (From Gregory & Prockop, 2007).

FIGURE 23. 5 Colony of iPS Cells FIGURE 23.5 Colony of iPS Cells. Typical iPS colony 25 days after infection. (a) Dark field, low magnification; (b) higher magnification of edge of colony outlined with dotted line.