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From: Conjunctivally Applied BDNF Protects Photoreceptors from Light-Induced Damage Trans. Vis. Sci. Tech.. 2015;4(6):1. doi:10.1167/tvst.4.6.1 Figure Legend: Topical eye application of BDNF increases BDNF level in the retina and optic nerve. We applied one single drop (10 μL) of different solutions (NaCl 0.9%, TSP 0.25%, CMC 0.2%) containing BDNF (12 μg/μL BDNF) at the level of conjunctival fornix in Wistar rats; the contralateral eye was treated with different solutions used for preparing BDNF. To increase the viscosity of solution, we used sodium carboxymethyl cellulose (CMC, 0.2%) and the polysaccharide fraction of TSP (0.25%). Our results show that single drop application of BDNF/TSP (12 μg/μL BDNF in 0.25% TSP) induced a significant increase of BDNF level in the retina (A) and optic nerve (B), compared to control vehicle treated eyes, BDNF/NaCl and BDNF/CMC topical eye treatment (n = 5 retinas in each group, *P < 0.05). Retinal level of BDNF was determined by ELISA 6 hours after treatment; *P < 0.05 (Student's t-test). Date of download: 10/6/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.