Accelerated wound healing through the incorporation of basic fibroblast growth factor- impregnated gelatin microspheres into artificial dermis using a pressure-induced decubitus ulcer model in genetically diabetic mice  Katsuya Kawai, Shigehiko Suzuki, Yasuhiko Tabata, Yoshihiko Nishimura  British Journal of Plastic Surgery  Volume 58, Issue 8, Pages 1115-1123 (December 2005) DOI: 10.1016/j.bjps.2005.04.010 Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 1 bFGF-impregnated gelatin microspheres with a water content of 96.75vol%. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 2 Scanning electron microscopic view of an artificial dermis containing gelatin microspheres. The average pore size of the artificial dermis was 70–110μm and the gelatin microsphere average diameter was 60μm. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 3 The ischaemic lesion 5 days after completion of the pressure load. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 4 The necrotic tissue was resected. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 5 The artificial dermis was implanted into the wound. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 6 Tissue appearance of the wound surface, 7 days after implantation of the artificial dermis. (A) Control group, (B) single application group and (C) sustained release group. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 7 Histological sections of artificial dermis 7 days after implantation. (A) Control group, (B) single application group and (C) sustained release group. Haematoxylin and eosin, original magnification ×100. The arrow heads indicate new capillaries in artificial dermis. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 8 Time course of fibroblast proliferation. Number of fibroblasts in the sustained release group (▪), the single application group (●), and the control group (▴). Each point shows the mean±SE (n=10). Student's t-test was used for all statistical analyses. *P<0.01, compared with the control group. †P<0.01, compared with the single application group. #P<0.01, compared with the control group. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

Figure 9 Time course of capillary proliferation. Number of capillaries in the sustained release group (▪), the single application group (●), and the control group (▴). Each point shows the mean±SE (n=10). Student's t-test was used for all statistical analyses. *P<0.01, compared with the control group. †P<0.01, compared with the single application group. #P<0.01, compared with the control group. British Journal of Plastic Surgery 2005 58, 1115-1123DOI: (10.1016/j.bjps.2005.04.010) Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions