Expression analysis of efflux pump genes among drug-susceptible and multidrug- resistant Mycobacterium tuberculosis clinical isolates and reference strains

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Expression analysis of efflux pump genes among drug-susceptible and multidrug- resistant Mycobacterium tuberculosis clinical isolates and reference strains Mustafa Kerem Calgin, Fikret Sahin, Buse Turegun, Devran Gerceker, Melike Atasever, Deniz Koksal, Djursun Karasartova, Mehmet Kiyan Diagnostic Microbiology and Infectious Disease Volume 76, Issue 3, Pages 291-297 (July 2013) DOI: 10.1016/j.diagmicrobio.2013.02.033 Copyright © 2013 Elsevier Inc. Terms and Conditions

Fig. 1 Efflux pump genes expression levels in DS (S1-S10) MDR M. tuberculosis (R1-R10) clinical isolates and reference strains (A, B, C). MRT-PCR of the total RNA of the clinical isolates and reference strains was carried out as described in Materials and methods, and products were analyzed on a 1.5% agarose gel. The gels were stained with EtBr, and EtBr signals were analyzed by computer-assisted densitometry. Results are presented as fold increases in the expression of target genes for each isolates. Diagnostic Microbiology and Infectious Disease 2013 76, 291-297DOI: (10.1016/j.diagmicrobio.2013.02.033) Copyright © 2013 Elsevier Inc. Terms and Conditions

Fig. 1 Efflux pump genes expression levels in DS (S1-S10) MDR M. tuberculosis (R1-R10) clinical isolates and reference strains (A, B, C). MRT-PCR of the total RNA of the clinical isolates and reference strains was carried out as described in Materials and methods, and products were analyzed on a 1.5% agarose gel. The gels were stained with EtBr, and EtBr signals were analyzed by computer-assisted densitometry. Results are presented as fold increases in the expression of target genes for each isolates. Diagnostic Microbiology and Infectious Disease 2013 76, 291-297DOI: (10.1016/j.diagmicrobio.2013.02.033) Copyright © 2013 Elsevier Inc. Terms and Conditions

Fig. 2 Relative expression profile of Rv1258c, DrrA, DrrB, IniA, MmpL7, Rv1250, Rv1634, Rv1273c, mmpL4, Rv0783c, Rv1687c, Rv2333c, Rv1410c, Rv0037c, Rv3000 in the groups of DS, MDR M. tuberculosis clinical isolates and the reference strains. The results from multiplex PCR showed higher mRNA levels in the DS and MDR M. tuberculosis clinical isolates compared to reference strains groups, and the difference was statistically significant. Diagnostic Microbiology and Infectious Disease 2013 76, 291-297DOI: (10.1016/j.diagmicrobio.2013.02.033) Copyright © 2013 Elsevier Inc. Terms and Conditions