Early polymerase chain reaction detection of Chagas disease reactivation in heart transplant patients Priscilla Almeida da Costa, MS, Marcela Segatto,

Slides:

Advertisements

Similar presentations

Systolic blood pressure on discharge after left ventricular assist device insertion is associated with subsequent stroke Michael E. Nassif, MD, Anjan Tibrewala,

Advertisements

Sixth INTERMACS annual report: A 10,000-patient database James K. Kirklin, MD, David C. Naftel, PhD, Francis D. Pagani, MD, PhD, Robert L. Kormos, MD,

Janet T. Lee, MD, Rosemary F. Kelly, MD, Marshall I

De novo donor-specific HLA antibodies are associated with early and high-grade bronchiolitis obliterans syndrome and death after lung transplantation

Table 5 Characteristics of 12 patients who had 1 test of stool samples that yielded positive results in the prospective clinical assessment for investigation.

Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil Kiev Martins, Cléber de Mesquita Andrade, Andressa.

Incremental and independent value of cardiopulmonary exercise test measures and the Seattle Heart Failure Model for prediction of risk in patients with.

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene Alessandro Saluto, Alessandro.

Therapeutic and Diagnostic Applications of Minor Histocompatibility Antigen HA-1 and HA-2 Disparities in Allogeneic Hematopoietic Stem Cell Transplantation:

Limitations of right heart catheterization in the diagnosis and risk stratification of patients with pulmonary hypertension related to left heart disease:

Oral mucosa as a source of Mycobacterium leprae infection and transmission, and implications of bacterial DNA detection and the immunological status

RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues Annunziata Gloghini, Barbara Canal, Ulf Klein, Luigino Dal Maso,

Natalia Kholod, Jacques Boniver, Philippe Delvenne

Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction O. Kupferschmidt, D. Krüger, T.K. Held, H. Ellerhrok,

Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR A. Heininger,

Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis Jeanne A. Jordan, Mary Beth.

Rafael Marques da Silva, DDS, Dominique A. Caugant, PhD, Emenike R. K

Detection of fungal DNA in lysis–centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients L. Trovato, P. Betta, M.G.

T790M EGFR Mutation Detection in Cerebrospinal Fluid and Response to Osimertinib in a Lung Cancer Patient with Meningeal Carcinomatosis Hugo Gortais,

M. Cogliati, R. D'Amicis, A.M. Tortorano

ALK FISH and IHC: You Cannot Have One without the Other

Detection of Colorectal Cancer Using a Simplified SEPT9 Gene Methylation Assay Is a Reliable Method for Opportunistic Screening Dong Wu, Guangpeng Zhou,

Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis ShriHari S. Kadkol, Annette Bruno, Carol Dodge,

IgH PCR of Zinc Formalin-Fixed, Paraffin-Embedded Non-Lymphomatous Gastric Samples Produces Artifactual “Clonal” Bands Not Observed in Paired Tissues.

A bovine protocol for training professionals in preimplantation genetic diagnosis using polymerase chain reaction Carlos Gilberto Almodin, M.D., Ph.D.,

Prevalence of diarrheagenic Escherichia coli strains detected by PCR in patients with travelers' diarrhea M. Vargas, J. Gascón, F. Gallardo, M. T. Jimenez.

Comparison of Allelic Discrimination by dHPLC, HRM, and TaqMan in the Detection of BRAF Mutation V600E Pablo Carbonell, María C. Turpin, Daniel Torres-Moreno,

Integron-mediated antibiotic multiresistance in Acinetobacter baumannii clinical isolates from Spain J. Ruiz, M.M. Navia, C. Casals, J.M. Sierra, M.T.

Detection of Central Nervous System Leukemia in Children with Acute Lymphoblastic Leukemia by Real-Time Polymerase Chain Reaction Sharon R. Pine, Changhong.

Molecular Diagnostic Approach to Non-Hodgkin's Lymphoma

Long-Range (17.7 kb) Allele-Specific Polymerase Chain Reaction Method for Direct Haplotyping of R117H and IVS-8 Mutations of the Cystic Fibrosis Transmembrane.

Acquired BRAF V600E Mutation as Resistant Mechanism after Treatment with Third- Generation EGFR Tyrosine Kinase Inhibitor Alessandra Bearz, MD, Elisa.

T790M EGFR Mutation Detection in Cerebrospinal Fluid and Response to Osimertinib in a Lung Cancer Patient with Meningeal Carcinomatosis Hugo Gortais,

Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis Juliana Polisseni, M.S., Wanderlei Ferreira de.

Analysis of human DNA present in the digestive tract of Aedes aegypti mosquitoes for possible forensic application B.R.C. Vieira, E.F. Carvalho, D.A.

M. J. Röβler, G. Rappl, M. Muche, D. O. Hasselmann, W. Sterry, W

PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia M. Dendis, R. Horváth, J. Michálek, F. Růžička,

Specific Detection of Cytokeratin 20-Positive Cells in Blood of Colorectal and Breast Cancer Patients by a High Sensitivity Real-Time Reverse Transcriptase-Polymerase.

Hereditary angioedema: Molecular and clinical differences among European populations Matthaios Speletas, MD, PhD, Agnes Szilagyi, PhD, Fotis Psarros,

PCR Analysis of the Immunoglobulin Heavy Chain Gene in Polyclonal Processes Can Yield Pseudoclonal Bands as an Artifact of Low B Cell Number Kojo S.J.

Rhodococcus equi pneumonia in a heart transplant recipient in Korea, with emphasis on microbial diagnosis S.J. Yoo, H. Sung, J.D. Chae, M. -N. Kim, C.H.

Sean C. Murphy, Daniel R. Hoogestraat, Dhruba J

Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis Jeanne A. Jordan, Mary Beth.

An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene Alessandro Saluto, Alessandro.

Rapid Detection of Clonal T-Cell Receptor-β Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve.

Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR A. Heininger,

Catherine E. Keegan, Anthony A. Killeen

Screening for Expanded Alleles of the FMR1 Gene in Blood Spots from Newborn Males in a Spanish Population Isabel Fernandez-Carvajal, Paulina Walichiewicz,

Analytical Detection of Immunoglobulin Heavy Chain Gene Rearrangements in Gastric Lymphoid Infiltrates by Peak Area Analysis of the Melting Curve in the.

A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V K. Boye, M.D. Bartels, I.S. Andersen, J.A. Møller,

Critical Evaluation of Real-Time Reverse Transcriptase-Polymerase Chain Reaction for the Quantitative Detection of Cytokeratin 20 mRNA in Colorectal Cancer.

Validation of the SHOX2/PTGER4 DNA Methylation Marker Panel for Plasma-Based Discrimination between Patients with Malignant and Nonmalignant Lung Disease

Single nucleotide polymorphism-based molecular typing of M

Detection of human telomerase reverse transcriptase mRNA in cells obtained by lavage of the pleura is not associated with worse outcome in patients with.

Gerda T. Noordhoek, Sitha A. Scheltinga, Paul Caesar, Leo M

Clinical Characteristics and Outcomes for Patients With Thymic Carcinoma: Evaluation of Masaoka Staging Anya M. Litvak, MD, Kaitlin Woo, MS, Sara Hayes,

Diagnosis of Trypanosomatid Infections

Chlamydia trachomatis and human papillomavirus infection in Indian women with sexually transmitted diseases and cervical precancerous and cancerous lesions

A Rapid, Sensitive Assay to Detect EGFR Mutation in Small Biopsy Specimens from Lung Cancer Yasushi Yatabe, Toyoaki Hida, Yoshitsugu Horio, Takayuki.

Rapid and Sensitive Real-Time Polymerase Chain Reaction Method for Detection and Quantification of 3243A>G Mitochondrial Point Mutation Rinki Singh,

T Cell Receptor γ-Chain Gene Polymerase Chain Reaction to Diagnose Central Nervous System Involvement by Cutaneous T Cell Lymphoma Robert Taylor, Jo-Anne.

Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants

Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas Qiulu Pan, William Pao, Marc.

ERCC1 mRNA Expression Is Not Associated with Response and Survival after Platinum-Based Chemotherapy Regimens in Advanced Non-Small Cell Lung Cancer

S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

Pyrosequencing for rapid identification of carbapenem-hydrolysing OXA-type β- lactamases in Acinetobacter baumannii T. Naas, L. Poirel, P. Nordmann Clinical.

Evaluation of diagnostic tests

Genomic, serologic, and clinical case-control study of Chlamydia pneumoniae and peripheral artery occlusive disease Jose P. Linares-Palomino, MD, PhD,

Detection and identification of bacterial DNA in semen

Presentation transcript:

Early polymerase chain reaction detection of Chagas disease reactivation in heart transplant patients Priscilla Almeida da Costa, MS, Marcela Segatto, PhD, Danielle Fernandes Durso, PhD, Wagson José de Carvalho Moreira, BSc, Lucas Lodi Junqueira, MD, PhD, Fábio Morato de Castilho, MD, MS, Silvio Amadeu de Andrade, MD, Cláudio Léo Gelape, MD, PhD, Egler Chiari, PhD, Andréa Teixeira-Carvalho, PhD, Sergio Danilo Junho Pena, MD, PhD, Carlos Renato Machado, PhD, Gloria Regina Franco, PhD, Geraldo Brasileiro Filho, MD, PhD, Maria da Consolação Vieira Moreira, MD, PhD, Andréa Mara Macedo, PhD The Journal of Heart and Lung Transplantation Volume 36, Issue 7, Pages 797-805 (July 2017) DOI: 10.1016/j.healun.2017.02.018 Copyright © 2017 International Society for the Heart and Lung Transplantation Terms and Conditions

Figure 1 Representative results of the PCR techniques. (A) Amplification of variable 330/660-bp regions of the T cruzi kDNA minicircle from endomyocardial biopsies of heart transplant patients: 1 to 12: EMB specimens of patients, where 4 showed positive amplification products; 13: DNA from T cruzi JG strain (positive control); 14: no DNA template (negative control). MW, standard molecular weight (1-kb Plus DNA Ladder; Invitrogen). (B) Dissociation curves obtained by the melting curve program for the rDNA 24Sα gene from biopsies of heart transplant patients. (I) Unspecific peaks obtained from negative EMB specimens in contrast to negative controls (without DNA). (II) Typical peaks derived from T cruzi DNA of JG (TcII) and Col1.7G2 (TcI) populations (positive controls). (III) Peaks obtained from positive EMB specimens in contrast to positive controls. The Journal of Heart and Lung Transplantation 2017 36, 797-805DOI: (10.1016/j.healun.2017.02.018) Copyright © 2017 International Society for the Heart and Lung Transplantation Terms and Conditions

Figure 2 Comparison of reactivation results between clinical and PCR molecular diagnostic procedures using EMB specimens (paraffin-embedded or fresh biopsies) from heart transplant patients with chagasic cardiopathy. (1) aTrue positive; bfalse positive; cfalse negative; dtrue negative; (2) N = 58; (3) total number of positive tests (a + b) = 39; (4) total number of clinical reactivations (a + c) = 38. The Journal of Heart and Lung Transplantation 2017 36, 797-805DOI: (10.1016/j.healun.2017.02.018) Copyright © 2017 International Society for the Heart and Lung Transplantation Terms and Conditions

Figure 3 ROC curves for different diagnosis approaches compared with clinical reactivation of Chagas disease. (A) Molecular diagnosis and histopathologic analysis of 500 EMB specimens from 58 patients (B) Molecular diagnosis, histopathologic analysis, blood smear and hemoculture of samples from 29 patients. Criterion >0 (positive = 1, negative = 0); AUC, area under the receiver operating characteristic (ROC) curve (p ≤ 0.001). The Journal of Heart and Lung Transplantation 2017 36, 797-805DOI: (10.1016/j.healun.2017.02.018) Copyright © 2017 International Society for the Heart and Lung Transplantation Terms and Conditions

Figure 4 Proposed algorithm for managing chagasic patients submitted to EMB. Number of patients associated to each box represents the number of cases observed in the present study. CDR, Chagas disease reactivation; EMB, endomyocardial biopsy. The Journal of Heart and Lung Transplantation 2017 36, 797-805DOI: (10.1016/j.healun.2017.02.018) Copyright © 2017 International Society for the Heart and Lung Transplantation Terms and Conditions