A rare case of lung coinfection by Streptomyces cinereoruber and Haemophilus influenzae in a patient with severe chronic obstructive pulmonary disease:

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A rare case of lung coinfection by Streptomyces cinereoruber and Haemophilus influenzae in a patient with severe chronic obstructive pulmonary disease: characterization at species level using molecular techniques Angel Manteca, Ana Isabel Pelaez, Maria del Mar Garcia-Suarez, Elisa Hidalgo, Benigno del Busto, Francisco J. Mendez Diagnostic Microbiology and Infectious Disease Volume 60, Issue 3, Pages 307-311 (March 2008) DOI: 10.1016/j.diagmicrobio.2007.10.009 Copyright © 2008 Elsevier Inc. Terms and Conditions

Fig. 1 Computed tomography imaging showing cylindrical bronchiectasis (arrows) in the middle and inferior portions of both lungs. Thickening of the bronchial wall on the left (A), as well as on the right (B), is observed. Bullous paraseptal emphysema measuring 12 cm is visible in the middle of the right lobe (arrowhead) (C). Diagnostic Microbiology and Infectious Disease 2008 60, 307-311DOI: (10.1016/j.diagmicrobio.2007.10.009) Copyright © 2008 Elsevier Inc. Terms and Conditions

Fig. 2 (A) i and ii, colonies of the Streptomyces isolate developed on glucose–asparagine–yeast extract medium obtained from swabbed pus (culture for 3 days at 30 °C). iii, detail of the hyphae under phase contrast microscopy. (B) RFLP of the Streptomyces isolate, S. cinereoruber ATCC19740 and S. lipmanii ATCC19779. i, 0.8% agarose gel electrophoresis. The Streptomyces spp. and restriction enzyme used are indicated at the top. Lane M, λ DNA cleaved with PstI. Molecular sizes are shown on the left. ii and iii, densitometric analysis of the gel. DNA gel images were imported into Scion Image (Beta 2 version for Windows 95, Scion Corp., Frederick, MD) for digital analysis. Plots of pixel intensity were obtained by positioning linear selections perpendicular to the gel lanes and by measuring pixel intensities along the linear selections. ii, comparison of the Streptomyces isolated and S. cinereoruber ATCC19740; iii, comparison of the Streptomyces isolated and S. lipmanii ATCC19779. Diagnostic Microbiology and Infectious Disease 2008 60, 307-311DOI: (10.1016/j.diagmicrobio.2007.10.009) Copyright © 2008 Elsevier Inc. Terms and Conditions