Figure 1. Scheme and photographs of the container for vitrification using the ‘straw in straw’ mode: (1) 0.5 ml straw; (2) metal closing ball; (3) open-pulled.

Slides:

Advertisements

Similar presentations

1 Vol. 01. p Vol. 01. p Vol. 01. p.20.

Advertisements

1 Vol. 03. p Vol. 03. p Vol. 03. p.21.

1 Vol. 02. p Vol. 02. p Vol. 02. p.19.

1 Vol. 03. p Vol. 03. p Vol. 03. p.16.

1 Vol. 02. p Vol. 02. p Vol. 02. p.30.

1 Vol. 03. p Vol. 03. p Vol. 03. p.35.

1 Vol. 02. p Vol. 02. p Vol. 02. p.10.

1 Vol. 01. p Vol. 01. p Vol. 01. p.14.

M M M M 5. Not Listed

Figure 1 WBC count (A), platelet count (B), MPV (C), platelet activation rate (D) and TGF-β1concentration (E) in the PF of women with endometriosis and.

Figure 3. Day 2 cell symmetry

E = m Lf E = m c θ E = m Lv.

Values of n for transvaginal ultrasonography scans, serum hormone measurements, and CPFM results on the respective cycle days relative to ovulation (n.

Figure 1 Cumulative chance of pregnancy according to age at initiation of pregnancy seeking. The curves were drawn based on the following assumptions:

Figure 2. AFMSCs differentiate into neurons

Volume by Water Displacement

Figure 1 Flow chart showing the selection of publications identified in the literature search. From: GnRH antagonist versus long agonist protocols in IVF:

Figure 1. The solution of the Faddy–Gosden differential equation for the primordial follicle population from birth to menopause. The primordial follicle.

Figure 1. Different factors that influence the higher cost of multiple pregnancies compared to singletons. PIH=pregnancy induced hypertension. HELLP=hemolysis,

Figure 1 Flow chart of search and selection strategy for studies in a systematic review of endometrial thickness during IUI with ovarian stimulation. *

Figure 1: Time points at which sperm samples were analysed for aneuploidy frequencies in controls and cancer patients From: Sperm aneuploidy frequencies.

Figure 1 Flowchart for the study population.

Figure 2 Comparison of pregnancy outcomes between true NC-FET and modified NC-FET. Odds ratio (OR) adjusted for clinical pregnancy (OR 0.90, 95% CI 0.73–1.12)

Figure 1 PRISMA flow diagram illustrating the process of selection of studies for inclusion in the systematic review. From: How effective are weight-loss.

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Fig. 1 a) Juvenile calf oocytes after IVM; b) cleavage-stage embryos 96 h postinsemination; c) in vitro-developed blastocysts; and d) 4 male and 3 female.

Figure 4. In-vitro sperm binding to ovulated eggs

Figure 1. Scattergram of serum CA-125 levels in 775 consecutive patients who had been diagnosed with gynecologic disease by laparotomy or laparoscopy.

Fig. 1 Expansion of porcine cumulus cells

Figure 7 miRNA and mRNA gene expression changes in the Poor Group

From: Nerve Injury-related Autoimmunity Activation Leads to Chronic Inflammation and Chronic Neuropathic Pain Anesthes. 2013;118(2): doi: /ALN.0b013e31827d4b82.

Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification Gary D. Smith, Ph.D., Paulo C. Serafini,

Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification Yun-Xia Cao, M.D., Ph.D., Qiong Xing,

Cryoprotectants up-regulate expression of mouse oocyte AQP7, which facilitates water diffusion during cryopreservation Ya-Jing Tan, M.Sci., Yun Xiong,

IVM and gene expression of sheep cumulus–oocyte complexes following different methods of vitrification Bita Ebrahimi, Mojtaba Rezazadeh Valojerdi, Poopak.

Successful slush nitrogen vitrification of human ovarian tissue

Clinical application of oocyte vitrification: a systematic review and meta-analysis of randomized controlled trials Ana Cobo, Ph.D., César Diaz, M.D.

Claudia Messias Gomes, M. D. , Cristine Ane Silva E. Silva, M. S

Silke Klocke, Claudia Tappehorn, Georg Griesinger

Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM P Vanderzwalmen, F Ectors, L Grobet, Y Prapas, Y Panagiotidis,

Reproductive BioMedicine Online

Rapid freezing versus slow programmable freezing of human spermatozoa

L. Parmegiani, G. E. Cognigni, S. Bernardi, S. Cuomo, W. Ciampaglia, F

Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc.,

Effect of oocyte vitrification on deoxyribonucleic acid methylation of H19, Peg3, and Snrpn differentially methylated regions in mouse blastocysts Ke-Ren.

Ervin Macas, Min Xie, Sara Schaufelberger, Gabriele S

Separation of a pronucleus by premature cytokinesis: a mechanism for immediate diploidization of tripronuclear oocytes? Bernd E. Rosenbusch, Ph.D., Michael.

Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence Ze-Xu Jiao, M.D., Ph.D., Min Xu,

Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in.

Reading the Graduated Cylinder

Oocyte cryopreservation outcomes including pre-cryopreservation and post-thaw meiotic spindle evaluation following slow cooling and vitrification of human.

Vitrification of mouse embryos with super-cooled air

New methods for cooling and storing oocytes and embryos in a clean environment of −196°C Amir Arav, Yehudit Natan, Paolo Emanuele Levi-Setti, Francesca.

Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival rate and meiotic spindle competence

S.P. Leibo, Ph.D., Thomas B. Pool, Ph.D. Fertility and Sterility

Genotoxicity assessment of mouse oocytes by comet assay before vitrification and after warming with three vitrification protocols Anais Berthelot-Ricou,

Successful elective and medically indicated oocyte vitrification and warming for autologous in vitro fertilization, with predicted birth probabilities.

VITRIFICATION:OPEN SYSTEM- PRACTICES, PROS & CONS

Prospective cohort study of three- versus two-dimensional ultrasound for prediction of oocyte maturity Doron Shmorgun, M.D., Edward Hughes, M.D., Patrick.

Stomatal aperture. Stomatal aperture. A, Stomata of the different lines with (+) and without (-) ABA treatment as photographed during the experiments.

Fertility and Sterility

Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification Jack Y.J. Huang, M.D., Hai Ying.

Effect of choline-supplemented sodium-depleted slow freezing versus vitrification on mouse oocyte meiotic spindles and chromosome abnormalities Jack.

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in.

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. (A,B)

Yoko Kumasako, B. E. , Eiko Otsu, M. En. , Takafumi Utsunomiya, M. D

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Chapter 20 Assisted Reproductive Technologies

Presentation transcript:

Figure 1. Scheme and photographs of the container for vitrification using the ‘straw in straw’ mode: (1) 0.5 ml straw; (2) metal closing ball; (3) open-pulled straw; (4) vitrification medium with oocyte; (5) meniscus of vitrification medium. From: Aseptic technology of vitrification of human pronuclear oocytes using open-pulled straws Hum Reprod. 2005;20(2):492-496. doi:10.1093/humrep/deh605 Hum Reprod | Human Reproduction vol. 20 no. 2 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Figure 2. Development of oocytes after vitrification with direct cooling of open-pulled straws (OPS) in liquid nitrogen versus vitrification using cooling of OPS previously located in hermetically closed 0.5 ml straws. Superscripts a–b, c–d and e–f indicate significant differences (P < 0.05). From: Aseptic technology of vitrification of human pronuclear oocytes using open-pulled straws Hum Reprod. 2005;20(2):492-496. doi:10.1093/humrep/deh605 Hum Reprod | Human Reproduction vol. 20 no. 2 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Figure 3. The same 2PN oocyte before vitrification (1), and after warming: for 5 min (2 and 3 at a different focus), 6 h (4), 18 h (5), 30 h (6), 42 h (7), 66 h (8), 78 h (9), 96 h (10) and 96 h, stained by Hoechst 33342 (11). Bar=30 μm. From: Aseptic technology of vitrification of human pronuclear oocytes using open-pulled straws Hum Reprod. 2005;20(2):492-496. doi:10.1093/humrep/deh605 Hum Reprod | Human Reproduction vol. 20 no. 2 © European Society of Human Reproduction and Embryology 2004; all rights reserved