Volume 11, Issue 5, Pages (January 2009)

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Volume 11, Issue 5, Pages 527-533 (January 2009) Human bone marrow mesenchymal cells express NG2: possible increase in discriminative ability of flow cytometry during mesenchymal stromal cell identification Ilknur Kozanoglu, Can Boga, Hakan Ozdogu, Oktay Sozer, Erkan Maytalman, Ayse Canan Yazici, Feride Iffet Sahin Cytotherapy Volume 11, Issue 5, Pages 527-533 (January 2009) DOI: 10.1080/14653240902923153 Copyright © 2009 International Society for Cellular Therapy Terms and Conditions

Figure 1 (A) Human MSC are spindle-shaped, large flat cells. Original magnification ×40. (B) Cell death was determined by fluorescence-activated cell sorting analysis using 7-AAD. (C) MSC do not express hematopoietic markers CD34 and CD45. (D) Cell-surface Ag of CD73 and CD105 on MSC. Cytotherapy 2009 11, 527-533DOI: (10.1080/14653240902923153) Copyright © 2009 International Society for Cellular Therapy Terms and Conditions

Figure 2 (A) Cell-surface Ag of NG2 and CD105 on MSC. (B) NG2 is not expressed on uncultured BM cells. (C) NG2 has a similar expression rate compared with the other markers of MSC. (D) Negative control of Oil Red O staining of human MSC culture before adipogenic treatment. Original magnification ×40. (E) To evaluate MSC multipotentiality. Oil Red O staining of human MSC culture after adipogenic treatment, showing lipid droplets. Original magnification ×40. (F) Alizarin Red S staining of human MSC culture after osteogenic induction, showing calcium phosphate deposits. Original magnification ×40. Cytotherapy 2009 11, 527-533DOI: (10.1080/14653240902923153) Copyright © 2009 International Society for Cellular Therapy Terms and Conditions