Cell biology 1.1 Introduction to cells 1.2 Ultrastructure of cells

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Cell biology 1.1 Introduction to cells 1.2 Ultrastructure of cells 1.3 Membrane structure 1.4 Membrane transport 1.5 The origin of cells 1.6 Cell division 1 1

Introduction to cells Essential idea: The evolution of multicellular organisms allowed cell specialization and cell replacement. 2 2

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Introduction to cells According to the cell theory, living organisms are composed of cells. Exceptions to the cell theory (atypical examples): striated muscle, giant algae and aseptate fungal hyphae. 4 4

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Acetabularia 6

7

Introduction to cells Organisms consisting of only one cell carry out all functions of life in that cell. Name and (briefly) explain the functions of life. Consider the functions of life in unicellular organisms (Paramecium feeding, Chlamydomonas): 8 8

Paramecium 9 9

Chlamydomonas 10 10

Introduction to cells Surface area to volume ratio is important in the limitation of cell size. Multicellular organisms have properties that emerge from the interaction of their cellular components. 11 11

Introduction to cells Specialized tissues can develop by cell differentiation in multicellular organisms. Differentiation involves the expression of some genes and not others in a cell’s genome. 12 12

Introduction to cells The capacity of stem cells to divide and differentiate along different pathways is necessary in embryonic development and also makes stem cells suitable for therapeutic uses (stem cells trachea transplant). 13 13

Introduction to cells Understand the use of stem cells to treat Stargardt’s disease and Leukemia. 14 14

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Introduction to cells Appreciate the ethics of the therapeutic use of stem cells from specially created embryos, from the umbilical cord blood of a new-born baby and from an adult’s own tissues. 16 16

Ultrastructure of cells Essential idea: Eukaryotes have a much more complex cell structure than prokaryotes. 17 17

Ultrastructure of cells Prokaryotes have a simple cell structure without compartmentalisation. Eukaryotes have a compartmentalised cell structure (Cellsalive). Electron microscopes have a much higher resolution than light microscopes. 18 18

Ultrastructure of cells Understand the structure and function of organelles within exocrine gland cells of the pancreas and within palisade mesophyll cells of the leaf (Harvard cell animation). 19

Membrane structure Essential idea: The structure of biological membranes makes them fluid and dynamic. 20 20

Membrane structure Phospholipids form bilayers in water due to the amphipathic properties of phospholipid molecules. Membrane proteins are diverse in terms of structure, position in the membrane and function. 21

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Membrane structure Cholesterol is a component of animal cell membranes. Understand that cholesterol in mammalian membranes reduces membrane fluidity and permeability to some solutes. 23

Membrane structure Analyse evidence from electron microscopy that led to the proposal of the Davson-Danielli model. Analyse the falsification of the Davson-Danielli model that led to the Singer-Nicolson model. Freeze-etched electron microgaphs Structure of membrane proteins Fluorescent antibody tagging 25

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Membrane transport Essential idea: Membranes control the composition of cells by active and passive transport. 27 27

Membrane transport Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport (animations). • The fluidity of membranes allows materials to be taken into cells by endocytosis or released by exocytosis. Vesicles move materials within cells (Amoeba feeding). 28

Membrane transport Understand examples: structure and function of sodium–potassium pumps for active transport and potassium channels for facilitated diffusion in axons. Application of osmosis: Tissues or organs to be used in medical procedures must be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis. 29

The origin of cells Essential idea: There is an unbroken chain of life from the first cells on Earth to all cells in organisms alive today. 30 30

The origin of cells Cells can only be formed by division of pre- existing cells. The first cells must have arisen from non-living material. 31

The origin of cells Application of ideas: Evidence from Pasteur’s experiments that spontaneous generation of cells and organisms does not now occur on Earth. 32

Miller-Urey experiment 33

Deep sea vents 34

Membrane formation 35

A mechanism for inheritance 36

The origin of cells The origin of eukaryotic cells can be explained by the endosymbiotic theory. 37

Cell division Essential idea: Cell division is essential but must be controlled. 38 38

Cell division Mitosis is division of the nucleus into two genetically identical daughter nuclei. Chromosomes condense by supercoiling during mitosis. 39

Cell division Cytokinesis occurs after mitosis and is different in plant and animal cells. Prokaryotes divide by binary fission. 40

Cell division Interphase is a very active phase of the cell cycle with many processes occurring in the nucleus and cytoplasm. Cyclins are involved in the control of the cell cycle. 42

Cell division Mutagens, oncogenes and metastasis are involved in the development of primary and secondary tumours. Consider the correlation between smoking and incidence of cancers. 44