From: Blocking Endothelin-B Receptors Rescues Retinal Ganglion Cells from Optic Nerve Injury through Suppression of Neuroinflammation Invest. Ophthalmol. Vis. Sci.. 2012;53(7):3490-3500. doi:10.1167/iovs.11-9415 Figure Legend: Note the high incidence of colocalization of nuclear staining with DAPI and ETB receptors (center panels) at the crushed site where a dense cellular infiltration of cells expressing ETB receptors is seen, and GFAP immunoreactivity is almost absent. In the area surrounding the crushed site, immunoreactivity to GFAP (green) is intensified (right panels) where an immersion of cells between the fibrils can be seen. Bar = 100 μm. (C) Higher-magnification view of the crushed site (area C) stained with antibodies to rabbit anti-ETB receptors (Alexa 594-conjugated goat anti-rabbit IgG secondary antibody) and mouse anti-CD68 (FITC-conjugated goat anti-mouse IgG secondary antibody). Some of the cells that have infiltrated the crushed site are positively stained with both CD68 (green) and ETB receptor (red) antibodies. Bar = 100 μm. (D) Higher-magnification view of the edge of the crushed site from another optic nerve with prior treatment with PBS. The sample was stained with antibodies to mouse anti-GFAP (FITC-conjugated goat anti-mouse IgG secondary antibody) and rabbit anti-PCNA (Alexa 594-conjugated goat anti-rabbit IgG secondary antibody). Some of the GFAP-positive cells (green) are also positively stained with PCNA (red). Bar = 100 μm. Date of download: 10/8/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.