Biochemistry.

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Presentation transcript:

Biochemistry

Ch 2: chemistry basics Ch 3: Water Ch 4: Properties of Carbon Ch 5: Biological macromolecules

Elements in the Body 96.3% 3.7% Trace elements O, C, H, N Ca, P, K, S, Na, Cl, Mg Trace elements B, Cr, Co, Cu, F, I, Fe, Mn, Mo, Se, Si, Sn, V, Zn

Nucleus Protons Neutrons Electron cloud Electrons

Numbers Atomic number = number of protons Mass number: protons + neutrons Atomic mass: decimal number

Bohr Models P 36

Bonds Covalent Ionic Sharing electrons Between nonmetals Transfer of electrons Between metals and nonmetals

Hydrogen bond Is a polar attraction

Reactions

Water Cohesive Adhesive High specific heat Polar Hydrogen bonds Universal solvent

pH <7 acid: High [H+] >7 base: Low [H+] 7 neutral

Carbon Miller - Urey Experiment

Isomers Structural Cis-trans (geometric) Enantiomers

Structural isomers

cis-trans isomers

Enantiomers: mirror images

Hydroxyl: -OH (alcohols)

Carbonyl: C=O

Aldehydes:

Ketone

Carboxyl: COO- or -COOH

Amino: -NH2

Sulfhydryl: -SH (Thiols)

Phosphate: PO4

Methyl: CH3

Amide

Ether:

Carbon chain names Methyl (Form) Ethyl (Acet) Propyl Butyl Pentyl Hexyl

Compound name endings Alcohols: ol Aldehydes: al Ketones: one Sugar: ose Enzyme: ase

Dehydration synthesis

Hydrolysis

Carbohydrates Fuel Building material

Aldose and Ketose

Disaccharide 2 monosaccharides Glycosidic linkage Sucrose = glucose + fructose Maltose = glucose + glucose Lactose = glucose + galactose

Polysaccharides Storage: Starch Glycogen Structural: Cellulose Chitin

Lipids Energy storage Not water soluble Protection Cell membranes Hormones

Lipids Fats: glycerol + 3 fatty acids Saturated: Unsaturated: only single C-C bonds Animal origin Unsaturated: C=C double bonds Plant origin Hydrogenated vegetable oil Trans fats

Formation of ester Ester bond

Fats

Fats

Phospholipids Form bilayers Hydrophilic head: outside Hydrophobic tail: inside

Steroids Hormones Precurser is cholesterol Sex hormones Adrenal cortex hormones

Steroid skeleton

Proteins Structure and movement Transport Immunity Enzymes Hormones Receptors

Primary Protein Structure Linear chain of amino acids Linked by peptide bonds (dehydration synthesis)

Secondary Protein Structure Hydrogen bonding between atoms in the backbone a helix b sheet

Secondary Structure

Tertiary Structure Interaction of amino acid R groups (side chains) Disulfide bridges (cysteine) Hydrophobic interaction

Tertiary Structure

Quartenary Structure Multiple polypeptides aggregate Hemoglobin is a globular protein composed of 4 polypeptide chains

Denaturation Breakdown of protein shape due to: pH changes Heat Salt content Medium polarity change

Protein folding Chaperonins: assist in proper folding Alzheimers, Parkinson’s, and mad cow disease result from misfolded proteins

Nucleic acids Made of nucleotides DNA and RNA Phosphate Sugar (pentose) Nitrogenous base DNA and RNA

The nitrogenous bases Purines: double ring Pyrimidines: single ring Adenine Guanine Pyrimidines: single ring Cytosine Thymine (in DNA) Uracil (in RNA)