Figure 1 MALDI mass spectrum of 23mer-1 obtained using DABP as the matrix. The total quantity of this oligonucleotide loaded was 62.5 fmol. From: A matrix.

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Figure 1 MALDI mass spectrum of 23mer-1 obtained using DABP as the matrix. The total quantity of this oligonucleotide loaded was 62.5 fmol. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 2 UV-visible spectra of the three matrices used Figure 2 UV-visible spectra of the three matrices used. The concentration of each matrix was 3.0 × 10<sup>−5</sup> M. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 3 MALDI mass spectra of 23mer-2 (500 fmol), obtained by diluting a 10 μM oligonucleiotide stock solution with (a) 0.1 M, (b) 0.2 M and (c) 0.4 M of NaCl, respectively. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 4 MALDI mass spectra of d(T)<sub>6</sub> obtained using (a) DABP, (b) 3-HPA and (c) 2,4,6-THAP as the matrix, respectively. The quantity of d(T)<sub>6</sub> loaded was 1.4 pmol without adding extra NaCl to the sample solution. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 5 MALDI mass spectra of 23mer-2 (50 fmol) obtained by diluting stock solution in 1 M urea. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 6 MALDI mass spectra of (a) 70 amol of d(T)6; (b) 62 Figure 6 MALDI mass spectra of (a) 70 amol of d(T)6; (b) 62.5 fmol of 23mer-1; and (c) 12.5 fmol of 12mer-1 and 12mer-2. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 7 MALDI mass spectra of d(T)<sub>6</sub> obtained with matrices of (a) DABP, (b) 3-HPA and (c) 2,4,6-THAP. The quantity of the oligonucleotide loaded was 1.4 pmol. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 8 MALDI mass spectra of (a) 5 pmol of a mixture of 12 different oligonucleotides; (b) the expanded spectrum (a) in the mass region from 5465 to 5555 Da. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 9 MALDI mass spectra of an oligonucleotide mixture obtained with matrices of (a) DABP, (b) 2,4,6-THAP and (c) 3-HPA, respectively. The quantity of each oligonucleotide loaded was 500 fmol. From: A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Nucleic Acids Res. 2006;34(13):e94. doi:10.1093/nar/gkl509 Nucleic Acids Res | © 2006 The Author(s)  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.