MHC sequencing project GenBank submission A. 800 CDS accessions for alleles of non-IPD curated genes (GenBank) B. 190 gene sequence alleles of IPD non-classical genes (GenBank) Update to (A) to full gene sequences (GenBank) and alignments (GitHub)
MHC sequencing project
MHC sequencing project
MHC sequencing project 330 visits; referring sites
KIR Polymorphism Workshop
Dynamic interactions of KIR and HLA class I underlie significant pressure to evolve Paul J. Norman Parham Lab Stanford
KIR and HLA have co-evolved to diversify their interaction Computer modeling Phylogenetics Cross-species comparison Population Genetics HLA KIR Binding site Norman et al. 2013 PloS Genetics
Multiple binders/functions of HLA KIR LILR TCR + peptide (HLA-E) Computer modeling Phylogenetics Cross-species comparison Population Genetics HLA-C
But KIR only have one job.. (e.g.) 2DL1*022 evolved in response to high C2 frequency Hilton et al. 2015 PLoS Genetics; 11:1005439.
High-resolution KIR and HLA class I Genotypes KIR Polymorphism Workshop High-resolution KIR and HLA class I Genotypes
KIR Polymorphism Workshop KhoeSan Pygmy Hadza West African Africa Modern humans Out of Africa
KIR Polymorphism Workshop Bari et al. 2009 Blood Hilton et al. 2015 J. Immunol
KIR Polymorphism Workshop
KIR Polymorphism Workshop
KIR Polymorphism Workshop
Han HLA Class I haplotypes All of top 10 encode at least 2 KIR ligands
Except East Asian and South American
(has novel amino acids at two positively-selected residues) An unusual KIR allele shared by Māori, Papua New Guineans and Indigenous Australians (has novel amino acids at two positively-selected residues) Residue 166 Residue 20 2DL4 3DL1 2DS4 3DL2 *028 *086 *001 *002 Maori/PPNG Haplotype *001loh *002loh *00104 *00501 Australian Haplotypes Nemat-Gorgani et al. 2014 Immunogenetics Loh et al. in prep
KIR3DL1; F166 and Q20 enhance recognition of target HLA 3DL1 _L166 3DL1_F166 3DL1_F166_Q20 (*086) Relative activity Effector: Jurkat cells with KIR3DL1+CD3ζ Target: HLA transfected [Philippa Saunders]
KIR Polymorphism Workshop