From: Vascular Endothelial Growth Factor Expression and Signaling in the Lens Invest. Ophthalmol. Vis. Sci.. 2003;44(9):3911-3919. doi:10.1167/iovs.02-1226 Figure Legend: (A) Proteins extracted from five whole adult mouse lenses were immunoprecipitated with a monoclonal antibody to VEGFR2 and Western blot analysis conducted with an anti-VEGFR2 polyclonal antibody. The blot shows the results obtained with duplicate samples. In addition to the native receptor, bands of lower molecular weight were detected (arrowheads). This suggests that lens cells sequentially process the receptor to lower molecular mass forms. The large smear at ∼50 kDa is due to cross reactivity between the monoclonal antibody used for immunoprecipitation (IgG) and the peroxidase-labeled secondary antibody used for detection. (B) VEGFR2 was detected in P10 and adult lens epithelial and fiber cells by Western blot analysis. Multiple lower molecular mass bands were detected. The most abundant of these correspond to the lower molecular mass bands in (A) after immunoprecipitation. Pretreatment of the antibody with the immunizing peptide greatly reduced staining in all bands, supporting the view that these bands were due to proteolytic processing of the receptor. Fifteen micrograms of total protein was loaded in each lane. (C) VEGFR2 levels increased in lens fiber cells during postnatal development. Only bands corresponding to the full-length protein are shown in this view. Fifteen micrograms of total protein was loaded in each lane. Date of download: 10/8/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.