Enzymes Catalysis functional parts of enzymes

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Presentation transcript:

Enzymes Catalysis functional parts of enzymes evaluation of enzyme activity classification of enzymes regulation of enzyme activity in cells inhibitors of enzymes

Sucrose is very stable compound, but our enzymes can break it down quickly to generate ATP

Velocity acceleration rate for some enzymes

Mechanism of action

Induced fit model of enzyme action

Functional parts of enzyme molecule

The velocity of chemical reactions and Evaluation of enzyme activity

What is velocity?

Determination of velocity of enzymatic reaction

Velocity at different product concentraions (Michaelis-Menten curve)

Km for differrent enzymes

Linearization

Regulation of enzyme activity

Allosteric regulation

Feedback regulation

Regulation by covalent modification

Proteolytic attivation of enzymes

Dependence of enzyme activityon ph and temperature

pH optimum for different enzymes

Temperature

inhibitors

Competitive inhibitor

Noncompetitive inhibitor

Anti-HIV drugs inhibit HIV protease

HIV genome and protease

Sulfanilamides (analogs of p-aminobenzoate) for treatment of bacterial infections: inhibitors of synthesis of folic acid Folic acid

Folic acid antagonists: antifolates as antitumor agents

Allopurinol inhibits purines breakdown and is used for treatment of gout

Viral infections can be treated using different thymidine analogs AZT Idoxuridine

Statins: inhibition of cholesterol biosynthesis for atherosclerosis treatment

Atorvastatin: powerful synthetic statin