HARVARD APPARATUS REGENERATIVE TECHNOLOGY ∙ ADVANCES IN REGENERATIVE MEDICINE An Advanced 3D Bioreactor Architecture for Decellularization and Recellularization of Solid Organ Constructs INTRODUCTION The four phases of regenerating organs ( explained below) requires a 3D bioreactor to be very versatile in its ability to carry out experimental environments in the 3D organ bioreactor as diverse as adherence of cells to the scaffold then switch to organ perfusion mode, then to perfusion and growth mode. This requires advanced control and acquisition as well as process data logging and feed-back control and versatile 3D bioreactor. The ORCA was designed To be able to switch its control and acquisition to adapt to the various demands of conditioning, decellurizing, recellurizing or natural or synthetic scaffolds.. Initiate database and data logging for GMP compatibility ORCA CONTROLLER (Organ Control & Acquisition System ) SUMMARY: The Hart ORCA series of 3D organ bioreactors is ideal for handling a complex decellurization and then transform itself into a recellurization 3D organ bioreactor, then sustain the organ and deliver it in perfect condition. Full diagnostic and operations monitoring Pump monitoring and control Image and sensor control and monitoring ABOVE: Total system solution for DECELLRIZATION and RECELLURIZTION of solid organs. ORCA IS SOFTWAE DRIVEN BY PROTOCOLS YOU DESIGN. TO MAKE THE 3D ORGAN BIOREACTOR RESPOND THE WAY IT SHOULD FOR THE PHASE YOU ARE IN Automation allows you 24 hour operation remaining sterile for weeks Automatic complex method- just touch a button DISCUSSION Highlights of the ORCA system are below. It has features of a process control systems and those of and acquisition control station. The advanced diagnostics allow for remote control with permission and notifications on your email or smart phone For status updates and error notification. These events are all data logged for historical record keeping. MATERIALS AND METHODS ORCA can store complex methods directly controlling: pumping, temperature, sheer force, pressure, ventilation And solvent exchanges,. This can be done by time, event or result. There are three solid organ bioreactors 33L 12 L and 2L, these are made of medical grade polycarbonate and can be autoclaved for sterilization. They have convenient ports for sensor placement , catheters and fluidic line, capable of visualizing with ultra sound, microscopes, Fluorescence and Infrared. ABOVE: 24 hour complete automation. UPS back-up- 6 hours without power 84 October Hill Road ∙ Holliston, MA 01746 USA ∙ toll-free: 774.233.7300 ∙ phone: 774.233.7301 ∙ fax: 774.233.7302 ∙ email: cservice@HARTregen.com www.HARTregen.com