Approach Extract all annotated exons (Refseq and KnownGene) in region plus buffer (200KB up & down stream. Extract all conserved elements with conservation.

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Presentation transcript:

Approach Extract all annotated exons (Refseq and KnownGene) in region plus buffer (200KB up & down stream. Extract all conserved elements with conservation score no lower than the average exon conservation score. Generate a primer tailing of the target elements tailored to the sequencing read size provided by the sequencing technology used.

DISC1/TSNAX Region Size: 500KB 22 Exons 4KB bases 300 non exonic conserved elements spanning 20KB Approx number of primer pairs 200 base read length 500 800 base read 400

DISC1/TSNAX

DISC1/TSNAX Exon Size Distribution

Neuregulin 1 Largest Isoform 1.1 MB 17 annotated exons spanning 6KB 850 non exonic conserved elements spanning 73KB. Approx number of primer pairs 200 base read length 1500 800 base read 1000

NRG1

NRG1 Exons