NATIONAL ANIMAL GENOME RESEARCH PROJECT NRSP-8

NRSP-8 Initiated: October, 1993 Revised: October, 1998 Termination (scheduled): October, 2003 Revision in progress

NRSP-8 Participants in current project: SAES: 21 States USDA/ARS: Four Locations Non-AES Universities: Four Commodity Assn: Three Private Industry: Five Foreign Countries: Three

NRSP-8 Administrative Advisors: North Central Margaret Dentine--Lead South: Jerry Cherry North East: Bill Trumble West: Colin Kaltenbach CSREES: Richard Frahm

NRSP-8 OBJECTIVES: 1. Develop high resolution comparative genome maps across species that link agricultural animal maps to those of the human and mouse genome

NRSP-8 OBJECTIVES: 2. Increase the marker density of existing linkage maps used in QTL mapping and integrate them with physical maps of animal chromosomes

NRSP-8 OBJECTIVES: 3. Expand and enhance internationally shared species genome databases and provide other common resources that facilitate genome mapping

NRSP-8 FY 2002 Budget MRF Funding $380,000 Matching/Cost Sharing $456,000 Total $836,000

NRSP-8 Species coordinators: Selected by competitive process Administer database management and provide centralized resources Present annual reports to researchers Respond to questions and requests at annual meeting during technical committee meetings Coordinate with international efforts

NRSP-8 Commodity Budget Coordinators Allocation Cattle (TX) $60,000 Swine (IA) $60,000 Poultry (MI) $50,000 Sheep (UT) $45,000 Horses (KY) $45,000 Databases ( IA, TX) $120,000

NRSP-8 Meetings/Communications Annual meeting in conjunction with Plant and Animal Genome Conference Coordinators communicate with participants via e-mail and web sites—data submitted to international databases

NRSP-8 Annual Publications (3 yr. avg): Refereed Journal Articles: 127 Other Publications: 136

NRSP-8 Brief Summary of Accomplishments: Cattle —Three major linkage maps with >3000 loci now published—Coordinator provides bovine reference DNA, fluorescent tagged and CATS primers and hybrid somatic cells to multiple laboratories

NRSP-8 Brief Summary of Accomplishments: Sheep—Database containing physical and linkage maps published and available-->450 microsatellite primer pairs mapped to ovine chromosomes have been distributed to more than 45 laboratories, worldwide for projects investigating wool traits, carcass composition, parasite resistance, growth characteristics, single gene defects, etc

NRSP-8 Brief Summary of Accomplishments: Swine —Over 650 genes and 1480 markers in swine database. Searchable swine EST database on line. Fluorescent primers prepared and shared with more than 40 laboratories world-wide—53 issues of Pig Genome Update have been published and shared widely

NRSP-8 Brief Summary of Accomplishments: Poultry—High resolution poultry genome consensus maps have been published with more than 20,000 markers. Large BAC libraries have been developed and a searchable genome mapping data base has been developed and is available on line

NRSP-8 Brief Summary of Accomplishments: Equine —Synteny, BAC and radiation hybrid panels have all been developed and are available for distribution—an active web site has been established to publicize and share information on horse genome mapping

NRSP-8 Brief Summary of Accomplishments: ANGENMAP website with public access http://www.genome.iastate.edu Has a discussion group (electronic list-serve) with 465 members worldwide. 1.4 million hits in 2001 Tutorials, access to all animal databases, reviews of software, searchable gene maps

NRSP-8 IMPACTS: Use of marker-assisted selection by genetic industry and private breeders initiated in most species

NRSP-8 IMPACTS: Identification of specific genes having a major impact on growth, carcass merit, reproductive efficiency and health will lead to improved production traits of all species at reduced costs allowing more competitiveness on the world market

NRSP-8 Conclusions: Use of these central resources provided by NRSP-8 has allowed many institutions to participate in genetic mapping effort for livestock improvement Feedback from users is built into the system of support for research Modest investment for widespread payoffs

NRSP-8 Rationale for Off-The-Top Funding  Animal genomics is a high priority  Funding is very limited  Central resources provide efficiencies of scale and leverage for national effort

NRSP-8 Rationale for Off-The-Top Funding-Con’t  Databases, primers and other materials would not have been available or available only in limited quantities were it not for NRSP-8  Most Agricultural Experiment Stations have benefited directly from the materials or information provided by NRSP-8